| 体外培养的小鼠子宫自然杀伤细胞与树突状细胞的相互影响 |
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| 引用本文： | 田玄玄,翁静. 体外培养的小鼠子宫自然杀伤细胞与树突状细胞的相互影响[J]. 生殖与避孕, 2010, 30(4): 223-229 |
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| 作者姓名： | 田玄玄 翁静 |
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| 作者单位： | 首都医科大学基础医学院组织学与胚胎学教研室生殖生物学实验室,北京,100069 |
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| 基金项目： | 北京市教委科技发展计划项目(项目号:KM200910025005) |
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| 摘 要： | 目的:探讨小鼠子宫自然杀伤细胞(uterine natural killer cells,uNK细胞)与树突状细胞(dendritic cells,DC)之间的相互影响。方法:①妊娠12~13d昆明小鼠子宫腺细胞区组织块培养后,分离出uNK细胞,单独培养(uNK组);②从小鼠骨髓分离得到未成熟DC,单独培养(DC组);③DC和uNK细胞直接接触共培养(共培养组)。观察各组细胞的生长状况,ELISA法检测培养上清液中IL-4、IL-10、IL-12、IL-15及IFN-γ的浓度,流式细胞术检测细胞表面CD40、CD86和CD83的表达。结果:与uNK组和DC组比,共培养组DC和uNK细胞生长聚集现象减少,2种细胞间相互接触,DC细胞表面CD40和CD86表达增高,但是CD83表达仍很低;培养上清液中IL-4、IL-10、IL-12、IL-15及IFN-γ的浓度均增加,尤以IL-4和IL-10增加显著。结论:uNK细胞和DC两者相互作用,DC参与介导uNK细胞的激活,促进其增殖,同时uNK细胞诱导DC呈部分激活状态,使其具有更好的耐受性。
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| 关 键 词： | 子宫自然杀伤细胞(uNK细胞) 树突状细胞(DC) 体外培养 |
Interactions between Cultured Uterine Natural Killer Cells and Dendritic Cells of Mouse |
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| Xuan-xuan TIAN,Jing WENG. Interactions between Cultured Uterine Natural Killer Cells and Dendritic Cells of Mouse[J]. Reproduction and Contraception, 2010, 30(4): 223-229 |
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| Authors: | Xuan-xuan TIAN Jing WENG |
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| Affiliation: | Xuan-xuan TIAN,Jing WENG (Laboratory of Reproductive Biology,Department of Histology , Embryology,School of Basic Medical Sciences,Capital Medical University,Beijing,100069) |
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| Abstract: | Objective: To investigate the influence between uterine natural killer cells (uNK cells) and dendritic cells (DC) of mice in vitro. Methods: 1) uNK cells migrated from blocks of the culturing metrial glands from pregnant mice on day 12-13 and cultured separately (uNK group). 2) Immature DC were originated from bone marrow and cultured separately (DC group). 3) DC and uNK cells co-cultured via direct contact (co-culture group). Cellular morphology was observed. The concentrations of IL-4, IL-10, IL-12, IL-15... |
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| Keywords: | uterine natural killer cells (uNK cells) dendritic cells (DC) culture in vitro |
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