膜联蛋白-1和巨噬细胞移动抑制因子在IgA肾病模型与人IgA肾病肾组织中的表达及其意义

目的探讨膜联蛋白-1(annexin1)和巨噬细胞移动抑制因子(MIF)在IgA肾病模型与人IgA肾病肾组织中的表达及在该病发展中的作用。方法用金黄色葡萄球菌(金葡菌)20肽抗原决定簇诱导IgA肾病模型的建立;选用人IgA肾病的肾活检组织。采用免疫组织化学技术检测上述两种肾组织。结果(1)annexin1在人IgAN肾组织和动物模型肾组织的表达强度与肾小管损伤程度均呈负相关,(P<0.01);(2)MIF在人IgA肾病肾组织和在动物模型肾组织的表达强度与肾小管损伤程度均呈正相关,(P<0.01)。结论annexin1在人IgAN的发展过程中起抑制作用,而MIF可起促进作用。annexin1和MIF在IgAN模型肾组织和人IgAN肾组织的表达的位置和强度均无差异。

Expression of Annexin-1 and MIF in IgAN Models Induced by 20 Peptide of Staphylococcus Aureus and IgAN Patients

Objective To investigate the expressions and functions of annexin-1 and macrophage migration-inhibitory(MIF)in IgAN model and patients.Methods Renal tissues from IgAN models induced by 20 peptide antigen epitope of Staphylococcus aureus and IgAN patients were detected with immunohistochemical method.Results(1)The expression intensity of annexin-1 in IgAN models and patients had negative correlation with the damage degree of renal tubules(P<0.01);(2)The expression intensity of MIF in IgAN models and patients had positive correlation with the damage degree of renal tubules(P<0.01=.Conclusion annexin-1 can inhibit,but MIF can promote the progression of IgAN.There is no remarkable difference on the expression sites and intensity of annexin-1 and MIF in IgAN models and patients.