重组人IFN-γ对感染弓形虫孕鼠细胞免疫及垂直传播的影响

目的观察重组人IFN-γ对感染弓形虫孕鼠细胞免疫状态及垂直传播的影响。方法取BALB/c孕鼠60只随机分为阳性感染对照组、治疗组及空白对照组。感染对照组与治疗组在孕鼠妊娠第8天用弓形虫速殖子攻击。治疗组于妊娠第7、8、9天用1000UIFN-γ治疗。所有各组孕鼠在妊娠第10、12天尾静脉采抗凝血,流式细胞仪检测CD4^＋、CD8^＋T细胞水平。并在妊娠第12天解剖孕鼠,观察活胎率及胎仔脑组织弓形虫感染情况。结果感染对照组及IFN-γ治疗组与空白对照组比,在孕后第10、12天CD4^＋T细胞水平显著降低,而CD8^＋T细胞水平显著升高,CD4^＋/CD8^＋T细胞比值倒置明显;但治疗组与感染对照组比较,治疗组在妊娠第10、12天CD4^＋T细胞显著升高,CD8^＋T细胞显著降低,CD4^＋/CD8^＋T细胞比值明显升高,活胎率也明显升高,宫内胚胎弓形虫感染率明显降低。结论IFN-γ治疗可以改善感染弓形虫孕鼠的细胞免疫功能,提高孕鼠免疫力,减少垂直传播概率。

Effect of rhu-IFN-&gamma|on immune state and vertical transmission in pregnant mice infected with Toxoplasma gondii

Objective To study the effect of rhu-IFN-γ on immune state and vertical transmission in pregnant mice infected with Toxoplasma gondii. Methods Sixty pregnant BALB/c mice were randomly divided into three groups： a control group, infected group and treatment group. In the infected group and treatment group, each mouse was injected with T. gondii tachyzoites peritoneally on the day 8 of gestation. In the treatment group, each mouse was treated with 1 000 U rhu- IFN-γ on the day 7, 8, 9 of gestation. Blood was collected from the tail veins of all the mice on the day 10, 12 of gestation. The levels of blood CD4^＋ and CD8^＋ T cells were detected by flow cytometry. Meanwhile, on the day 12 of gestation, all mice were anatomized to observe live embryo rate and the infection status in fetal brain tissue. Results Compared with the control group, the levels of CD4^＋ T cells in the infected group and treatment group were low, and the levels of CD8^＋ T cells high on the day 10, 12 of gestation, so the ratio of CD4^＋/CD8^＋ was inversed. However, compared with the infected group, the levels of CD4^＋ T cells in the treatment group were high and the levels of CD8^＋ T cells low on the day 10, 12 of gestation, so the ratios of CD4^＋/CD8^＋ were high on the day 10, 12 of gestation. Meanwhile, the live embryo rate was high and the infection rate of intrauterine embryonic low. Conclusion A proper dose of rhu-IFN-y could improve the function of immunity system and reduce the vertical transmission probability in pregnant mice infected with T. gondii.