用皮肤移植法对培育近交系大小鼠进行遗传监测

目的 采用皮肤移植技术对利用微卫星DNA监控所培育的近交系大小鼠进行遗传监测。方法利用背部皮肤移植法和尾部皮肤移植法同时对所培育的近交系大小鼠进行遗传监测。结果 对所培育12只大鼠的背部皮肤移植全部成活，没有排斥反应；12只小鼠的背部皮肤移植有1只出现技术失败，其余未出现排斥反应。对所培育12大鼠的尾部皮肤移植除有1只技术失败外，其他未出现排斥反应。小鼠的尾部皮肤移植有1只技术失败，1只死亡外，其他未出现排斥反应。结论 通过皮肤移植遗传监测初步证实了所培育的大小鼠群为近交系。

Genetic Monitoring of Cultivated Inbred Mice and Rats by Skin Transplantation

Objective To evaluate the value of skin transplantation and DNA microsatellites techniques in genetic monitoring of cultivated inbred mice and rats and to prove if the cultivated animals are of the same inbred strain. Methods Skin autotransplatation and allotransplantation were performed on the back and tail of the mice and rats. Results The allotransplanation of skin on the back of 12 rats was succedded without rejection response. The allotransplanted skin on the back of 12 mice survived in 11 and died in one mouse because of technical failure. The allotransplanted skin on the tail of 12 rats was alive in 11 and failed in one rat because of technical reasons. The allotransplanted skin on the tail of mice was alive in 10 mice, surgical failure in one mouse and one mouse died for undefined reason at 3 weeks after operation. No rejection response was observed in any rat or mouse with allotransplantion of back or tail skin at 100 days after operation. Conclusion The genetic monitoring by skin transplantation proved that those groups of cultivated rats and mice were of inbred strains.