人参皂苷Re对人神经母细胞瘤SH-SY5Y细胞缺氧复氧损伤的保护作用

目的：探讨人参皂苷Re 对人神经母细胞瘤SH-SY5Y 细胞缺氧复氧损伤的保护作用。 方法：采用缺氧复氧法制备SH-SY5Y 细胞损伤模型，分为对照组、模型组、Re 6.25 μg/mL 组和Re 12.50 μg/mL 组。采用MTT法测定细胞存活率，四甲基罗丹明甲酯（TMRM）染色评估细胞线粒体膜电位变化，DAPI 染色观察细胞凋亡率，并进一步采用免疫印迹检测各组细胞核因子NF-E2 相关因子2（Nrf-2）、Bax 和p53 的蛋白表达。 结果：人参皂苷Re 对SH-SY5Y 细胞活力无显著影响；缺氧复氧条件下，细胞存活率显著降低，而人参皂苷Re 在6.25、12.50 μg/mL 浓度下预保护可显著提高细胞存活率。缺氧复氧损伤后，人参皂苷Re 6.25、12.50 μg/mL 能显著提高SH-SY5Y 细胞的线粒体膜电位水平和抑制SH-SY5Y 细胞的凋亡率。人参皂苷Re 能够提高Nrf-2 的蛋白表达，抑制Bax 和p53 蛋白的表达。结论：人参皂苷Re 可以抑制缺氧复氧损伤细胞的凋亡和氧化损伤，对缺氧复氧诱导的神经细胞具有显著的保护作用。

Protective effect of ginsenoside Re on human neuroblastoma SH-SY5Y cellsinjured by hypoxia reoxygenation

Objective To observe the protective effect of ginsenoside Re on human neuroblastoma SH-SY5Ycells injured by hypoxia reoxygenation. Methods SH-SY5Y cells were divided into control group， hypoxiareoxygenation group， Re 6.25 μg/mL group， and Re 12.50 μg/mL group. MTT assay was used to determine the cellsurvival rate. Mitochondrial membrane potential was assessed using TMRM staining. DAPI staining was used toobserve cell apoptosis. And immunoblotting was used to detect the protein expression of nuclear factor-erythroid2-related factor 2（Nrf-2）， Bax and p53. Results Ginsenoside Re alone had no significant effect on the viability ofSH-SY5Y cells. The viability of SH-SY5Y cells was significantly decreased in the model group. While ginsenosideRe at 6.25 μg/mL and 12.50 μg/mL could significantly improve the survival rate of SH-SY5Y cells. Ginsenoside Re6.25 μg/mL and 12.50 μg/mL could significantly increase mitochondrial membrane potential and inhibit the apoptosisrate. Ginsenoside Re could increase the protein expression of Nrf-2 and inhibit the expression of Bax and p53.Conclusion Ginsenoside Re can inhibit cell apoptosis and oxidative damage and has significant protective effect onneurons induced by hypoxia reoxygenation