Root Canal Disinfection by Single- and Multiple-instrument Systems: Effects of Sodium Hypochlorite Volume,Concentration, and Retention Time |
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| Affiliation: | 1. Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Rio de Janiero, Brazil;2. Department of General Practice, University of Oslo, Oslo, Norway;1. Graduate Endodontics Program, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada;3. Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada;2. State Key Laboratory of Mechanics and Control of Mechanical Structures, Nanjing University of Aeronautics and Astronautics, Nanjing, China;4. Department of Dentistry, Mount Sinai Hospital, Sinai Health System, Toronto, Ontario, Canada;1. Department of Microbiology, School of Dentistry, University of Granada, Granada, Spain;3. Department of Stomatology, School of Dentistry, University of Granada, Granada, Spain;2. Department of Endodontics, Bauru Dental School, University of São Paulo, Bauru, São Paulo, Brazil;1. Department of Preventive, Restorative and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland;2. Laboratory of Oral Microbiology, Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland;1. Graduate Endodontics Program, University of Toronto, Faculty of Dentistry, Toronto, Ontario, Canada;2. Department of Restorative Dentistry, Dental School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;3. Department of Endodontics, University of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil |
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| Abstract: | ![]()
IntroductionThis ex vivo study evaluated the intracanal bacterial reduction promoted by chemomechanical preparation using a single-file technique varying the volume, concentration, and retention time of sodium hypochlorite (NaOCl) irrigation in comparison with a multifile system.MethodsPalatal roots from extracted maxillary first molars were selected and anatomically matched based on microcomputed tomographic analysis for group distribution. The canals were contaminated with a fresh mixed bacterial culture grown in anaerobiosis and recently obtained from a tooth with apical periodontitis. Specimens were divided into 4 groups of 24 each according to the following preparation protocols: REC-6LOW (Reciproc R50 instrument [VDW, Munich, Germany], 6% NaOCl, low irrigant volume), REC-2.5LOW (R50, 2.5% NaOCl, low irrigant volume), REC-2.5HI (R50, 2.5% NaOCl, high irrigant volume), and BR-2.5HI (BioRaCe [FKG Dentaire, LaChaux-de-Fonds, Switzerland], 2.5% NaOCl, high irrigant volume). The total time of preparation was recorded. Intracanal bacteriologic samples were taken before and after preparation; DNA was extracted and subjected to quantitative polymerase chain reaction.ResultsBacteria were detected in 22 initial samples from the REC-2.5LOW group and in 23 from the other groups. Intragroup analysis showed that all tested preparation protocols were highly effective in significantly reducing the intracanal bacterial counts (P < .001). Intergroup comparison of bacterial reduction levels revealed a statistically significant difference between BR-2.5HI and REC-2.5LOW (P < .05). Counts of bacteria were 2.5 times significantly higher in REC-2.5LOW compared with BR-2.5HI. No other significant differences were found in quantitative findings (P > .05).ConclusionsThe concerted effects of multiple instruments, the high volume of irrigation, and the long retention time of NaOCl irrigant had a positive influence on intracanal disinfection during chemomechanical preparation. |
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| Keywords: | Bacterial reduction endodontic treatment root canal irrigation root canal preparation sodium hypochlorite |
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