| Selection of the Mimic Epitopes of Antigens from Schistosoma japonicum by Using Phage Display Techniques |
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| 引用本文： | 王敏,易新元,曾宪芳,李先平,周东明,张顺科,章洁. Selection of the Mimic Epitopes of Antigens from Schistosoma japonicum by Using Phage Display Techniques[J]. 中华微生物学和免疫学杂志(英文版), 2003, 1(1): 91-94 |
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| 作者姓名： | 王敏 易新元 曾宪芳 李先平 周东明 张顺科 章洁 |
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| 作者单位： | Department of Clinical Laboratory，the Second Xiangya Hospital of Central South University，Changsha 410011，China，Department of Parasitology，Xiangya Medical College of Central South University，Changsha 410078，China，Department of Parasitology，Xiangya Medical College of Central South University，Changsha 410078，China，Department of Clinical Laboratory，the Second Xiangya Hospital of Central South University，Changsha 410011，China，Department of Parasitology，Xiangya Medical College of Central South University，Changsha 410078，China，Department of Parasitology，Xiangya Medical College of Central South University，Changsha 410078，China，Department of Parasitology，Xiangya Medical College of Central South University，Changsha 410078，China |
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| 基金项目： | This study was supported by grants from WHO/TDR (980255) and the Science Commission of Hunan Province (00jzy2115) |
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| 摘 要： | Rat was a semi permissive host of Schistosoma. Accordingto the research of Yu XC[1], when rat was infected withSchistosoma japonicum (S.j), the egg granulomas in ratlivers were rarely observed, while majority of eggs wereripe eggs and black dead eggs, and the number of meanliver eggs per gram (LEPG) was 1393.46, apparently lessthan that in permissive host mouse. These results indicatedapparently natural resistance. In our laboratory, enzyme linked immuno electro transfer blot (EITB) …
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| 关 键 词： | 噬菌体 日本血吸虫 免疫原 模拟短肽 模拟抗原 抗原肽 免疫抑制剂 免疫性 |
Selection of the Mimic Epitopes of Antigens from Schistosoma japonicum by Using Phage Display Techniques |
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| WANG Min YI Xinyuan ZENG Xianfang LI Xianping ZHOU Dongming ZHANG Shunke and ZHANG Jie. Selection of the Mimic Epitopes of Antigens from Schistosoma japonicum by Using Phage Display Techniques[J]. Chinese Journal of Microbiology and Immunology, 2003, 1(1): 91-94 |
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| Authors: | WANG Min YI Xinyuan ZENG Xianfang LI Xianping ZHOU Dongming ZHANG Shunke and ZHANG Jie |
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| Affiliation: | [1]DepartmentofClinicalLaboratory,theSecondXiangyaHospitalofCen-tralSouthUniversity,Changsha410011,China [2]DepartmentofParasitology,XiangyaMedicalCollegeofCentralSouthUniversity,Changsha410078,China |
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| Abstract: | To obtain short peptides simulating antigenic epitopes related to natural resistance against Schistosoma japonicum ( S .j) in rots, and to explore their immune protection against S .j in mice, phage random peptide library of 12 amino acids were screened with purified IgG from normal rat sere. Positive clones that were obtained after three rounds of bioparming were detected by EI,ISA, and two of them were sequenced. Kunming mice were immunized with mixed phage clones. Each mouse was challenged with 40±1 S.j cercariae, and all mice were perfused 45 days post-challenge. The worms and the liver eggs were counted. The results were that the specific phages binding to IgG were enriched 300 folds after three rounds of biopanning. Twenty clones were detected by ELISA and 19 of them bound to the specific IgG of rat sera. The sequence of two clones revealed no homology with other sequences in the GenBank. Compared with the control groups, the reduction rates of the worm burden and liver egg were 33.6% and 59.8%, respectively. It was concluded that the specific peptides,which simulate antigenic molecules correlated with natural resistance to S. j in rats could be obtained by immunosceening phage random peptide library and a protective immunity against S .j can be detected by these epitopes in mice. |
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| Keywords: | Shistosoma japonicum Antigenic peptides Immunoscreening Phage random peptide library |
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