HPLC同时测定心脑联通胶囊中葛根素、虎杖苷和野黄芩苷的含量

 目的利用梯度洗脱,建立高效液相色谱法测定心脑联通胶囊中葛根素、虎杖苷和野黄芩苷含量的方法。方法采用高效液相色谱法。Diamonsil ODS柱(4.6mm×200mm,5μm),流动相为乙腈(A)和0.05%磷酸水溶液(B)梯度洗脱,流速1.0m L·min^-1,检测波长336nm。结果葛根素的线性范围为0.0232～0.1856g·L^-1(r=0.9995),平均回收率为100.0%,RSD=1.3%,虎杖苷的线性范围为0.0152～0.1216g·L^-1(r=0.9996),平均回收率为98.7%,RSD=0.9%,野黄芩苷的线性范围为0.0046～0.0369g·L^-1(r=0.9998),平均回收率为100.4%,RSD=0.9%。结论本方法操作简便,测定结果准确可靠,可用于心脑联通胶囊的质量控制。

Simultaneous Determination of Puerarin,Polygonin and Scutellarin in Xinnaoliantong Capsules by HPLC

OBJECTIVE To develop a quantitative method simultanous for determination of puerarin,polygonin and scutellarin in Xinnaoliantong capsules by HPLC.METHODS The seperation was performed on a column Diamonsil ODS(4.6 mm×200 mm,5 μm) was used.The mobile phase was composed of acetonitrile(A) and 0.05% H₃PO₄(B) with gradient eluation.The flow rate was 1.0 mL·min^-1 with the wavelength at 336 nm.RESULTS For puerarin,the calibration curve was linear(r=0.999 5) in the range of 0.023 2-0.185 6 g·L^-1,the average recovery was 100.0%(RSD=1.3%).For polygonin,the calibration curve was linear(r=0.999 6) in the range of 0.015 2-0.121 6 g·L^-1,the average recovery was 98.7%(RSD=0.9%).For scutellarin,the calibration curve was linear(r=0.999 8) in the range of 0.004 6-0.036 9 g·L^-1,the average recovery was 100.4%(RSD=0.9%).CONCLUSION The method was sensitive,accurate and can be used in the quality control of Xinnaoliantong capsules.