钙预适应在心肌内源性保护机制中的应用

目的 观察钙离子变化对心肌保护作用的影响。方法 将５６只雄性ＳＤ大鼠随机分为７组，Ａ：缺血对照（ＩＣ）组；Ｂ：短暂无镍复钙灌流（ＣＰＣ）组，Ｃ：短暂缺血复灌（ＩＰＣ）组，Ｄ：短暂无钙复钙灌流十维拉帕米（ＣＰＣ＋Ｖｅｒａ）组，Ｅ：短暂缺血复灌十维拉帕米（ＩＰＣ＋Ｖｅｒａ）组；Ｆ：钙通道激动剂（Ｂａｙ Ｋ８６４４）组；Ｇ：Ｌ－型钙通道阻滞剂〖维拉帕米（ｖｅｒａｐａｍｉｌ）〗组。实验中观察缺血前及再灌后

The role of Ca2+ preconditioning in the mechanism of the myocardial endogenous protective effects

Objective To investigate the role of Ca 2 preconditioning in the mechanism of the myocardial endogenous protective effects.Methods Fifty six Sprague Dawley male rats were divided into 7 groups randomly:IC group,ischemic control;CPC group,hearts being perfused for 3 cycles of Ca 2 free and Ca 2 containing KH buffer;IPC group,ischemia followed by reperfusion; CPC Verapamil group;IPC Verapamil group;Bay K8644 group;Verapamil group.Heart function and coronary flow were examined before ischemia and after reperfusion.The release of LDH and the content of ATP were investigated and ultrastructural alterations in the myocardium were examined.Results Significant heart functional recovery rate was observed in IPC and CPC groups as compared with that in IC group(P<0.05).Administration of verapamil completely abolished the IPC and CPC induced cardiac protection.Administration of Bay K8644 could mimic cardiac protection against ischemia reperfusion injury.Conclusion IPC and CPC can induce myocardial endogenous protective effects against ischemia reperfusion injury.Administration of verapamil reduces Ca 2 influx and can mimic cardioprotection against ischemia reperfusion injury.