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| 游泳训练对大鼠腓肠肌铁代谢相关蛋白表达的影响 | |
| 引用本文： | 刘玉倩,常彦忠,钱忠明,刘建国,段相林. 游泳训练对大鼠腓肠肌铁代谢相关蛋白表达的影响[J]. 中国运动医学杂志, 2005, 24(2): 147-151 |
| 作者姓名： | 刘玉倩 常彦忠 钱忠明 刘建国 段相林 |
| 作者单位： | 1. 河北师范大学生命科学学院动物细胞生物学研究室,石家庄,050016 2. 河北师范大学生命科学学院动物细胞生物学研究室,石家庄,050016;香港理工大学应用生物及化学科技学系 3. 河北师范大学体育学院 |
| 基金项目： | 河北省自然科学基金，河北省教育厅博士科研项目，河北师范大学校科研和教改项目 |
| 摘 要： | 目的:研究游泳训练后大鼠腓肠肌二价金属离子转运体1(divalent metal transporter 1, DMT1)、转铁蛋白受体(transferrin receptor, TfR)及膜铁转运蛋白1(ferroportin1, FPN1)表达的变化,探究运动对腓肠肌铁代谢的影响机制.方法:40只雌性SD大鼠随机分为对照组和游泳训练组,分别于游泳训练5周及10周后取材,测定血清铁状态、腓肠肌非血红素铁含量,采用Western Blot方法检测腓肠肌铁代谢相关蛋白表达的变化.结果:(1)游泳训练5周及10周组大鼠血清铁、Hb及RBC均较对照组显著升高,游泳运动10周组大鼠腓肠肌非血红素铁含量显著增加(P<0.01);(2)游泳训练组大鼠腓肠肌DMT1(IRE)和TfR表达增加,FPN1表达减少(P<0.05),而DMT1(non-IRE)表达无明显变化.结论: 游泳训练可以增加腓肠肌非血红素铁含量,促进机体铁动员.这可能与腓肠肌细胞膜上向胞内转运铁的TfR和DMT1(IRE)蛋白表达增加,而向胞外转运铁的FPN1表达下降有关. |
| 关 键 词： | 腓肠肌 非血红素铁 膜铁转运蛋白1 二价金属离子转运体1 大鼠 |
| 修稿时间： | 2004-07-26 |
Effects of Swim Training on the Expression of Gastrocnemius'''' Iron Transport Proteins in Rats |
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| Liu Yuqian,Chang Yanzhong,Qian Zhongming ,,et al.. Effects of Swim Training on the Expression of Gastrocnemius'''' Iron Transport Proteins in Rats[J]. Chinese Journal of Sports Medicine, 2005, 24(2): 147-151 | |
| Authors: | Liu Yuqian Chang Yanzhong Qian Zhongming et al. |
| Affiliation: | Liu Yuqian1,Chang Yanzhong1,Qian Zhongming 1,2,et al. 1 Department of Animal Cytobiology,College of Life Science,Hebei Normal University,Shijiazhuang,China 050016 2 Laboratory of Iron Metabolism,Department of Applied Biology and Chemical Technology,The Hong Kong Polytechnic University,Kowloon,Hong Kong,PRC |
| Abstract: | Objective To investigate the effects of swim training on the expression of divalent metal transporter 1(DMT1), transferrin receptor(TfR) and ferroportin 1(FRN1) in rats' gastrocnemius. Methods 40 female SD rats were randomly divided into two groups: control group(CG)and exercise group(EG), and were sampled after 5 and 10-week swimming. Determined serum iron status and non-heam iron of gastrocnemius. Observed the expression of TfR, DMT1 and FPN1 by Western Blot. Results (1)The serum iron, Hb and RBC were significantly higher than those in control group. The gastrocnemius' non-heam iron of exercise group were significantly increased after 10-week training(P<0.01). (2) The expression of DMT1 (IRE) and TfR of exercise group was increased, but FPN1 expression was decreased, and DMT1 (non-IRE) had no change. Conclusion Suitable training could improve body iron homeostasis, increase gastrocnemius' non-heam iron. The mechanism of changes were that the expression of DMT1 (IRE) and TfR were increased but that of FPN1 was decreased. |
| Keywords: | gastrocnemius non-heam iron ferroportin 1 divalent metal transporter 1 rats$$ |
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