氯沙坦钾对糖尿病肾病大鼠肾组织p-JAK2、p-STAT3及VEGF表达的影响

目的：探讨氯沙坦钾对糖尿病肾病（diabetic nephropathy，DN）大鼠肾组织 p - JAK2、p - STAT3及 VEGF 表达的影响。方法：健康雄性 SD 大鼠30只随机分为正常对照组（C 组）、DN 模型组（DN 组）、氯沙坦钾组（L 组）。采用单次腹腔内注射链脲佐菌素（streptozotocin，STZ）法建立 DN 大鼠模型，周期12周。实验12周末检测大鼠血糖、Scr、BUN、24 h 尿蛋白定量；光镜及电镜下观察大鼠肾组织病理改变；采用免疫组化方法检测大鼠肾组织 p - JAK2、p - STAT3、VEGF 表达。结果：实验12周末，模型组大鼠血糖、Scr、BUN、24 h 尿蛋白定量均高于对照组（P ﹤0．05），肾组织中 p - JAK2、p - STAT3、VEGF 表达均高于对照组（P ﹤0．05）；氯沙坦钾组大鼠 Scr、BUN、24 h 尿蛋白定量均低于模型组（P ﹤0．05），肾组织 p - JAK2、p -STAT3、VEGF 表达均低于模型组（P ﹤0．05）。结论：氯沙坦钾可能部分通过调控 p - JAK2、p - STAT3及 VEGF 的表达而发挥肾脏保护作用。

Effect of Losartan Potassium on p - JAK2,p - STAT3 and VEGF of Renal Tissue in the Diabetic Nephropathy Rats

Objective：To investigate the effect of Losartan Potassium on p - JAK2,p - STAT3 and VEGF of renal tissue in the diabetic nephropathy rats. Methods：30 SD male rats were divided randomly into control group（group C）,diabetic nephropathy group（group DN）,Losartan Potassium group（group L）. The rats were being intraperitoneal injected with STZ（1% ,45 mg/ kg）to establish diabetic nephropathy rats models,test period 12 weeks. Blood glucose,Scr,BUN,24 - hour urinary protein were measured at the 12th. The renal pathological changes were detected by light microscope and electron microscopy. The expression of p - JAK2, p - STAT3 and VEGF were detected by immunohistochemistry staining sections. Results：After establishing mode for 12 weeks,com-pared with group C,group DN had significant elevated blood glucose,24 - hour urinary protein,Scr,BUN（P ﹤ 0. 05）,and immuno-histochemistry staining displayed that the expression of p - JAK2,p - STAT3,VEGF remarkably increased（P ﹤ 0. 05）. Compared with group DN,group L had significantly lower 24 urinary protein,Scr,BUN（P ﹤ 0. 05）,the expression of p - JAK2,p - STAT3, VEGF were lower than those in group DN（P ﹤ 0. 05）. Conclusion：Losartan Potassium may in part through regulating the expression of p - JAK2,p - STAT3 and VEGF play a protective role in the kidney of diabetic nephropathy rats.