| 人脐血间充质干细胞培养及其生物学特性的研究 |
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| 引用本文： | 扎拉嘎胡,买霞,陈晓,陈小义,陈莉. 人脐血间充质干细胞培养及其生物学特性的研究[J]. 解剖科学进展, 2009, 15(2) |
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| 作者姓名： | 扎拉嘎胡 买霞 陈晓 陈小义 陈莉 |
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| 作者单位： | 中国人民武装警察部队医学院,细胞生物学教研室,天津,300162;中国人民武装警察部队医学院,附属医院妇产科,天津,300162 |
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| 基金项目： | 武警部队科学基金资助项目 |
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| 摘 要： | 目的建立人脐血间充质干细胞体外分离培养的最佳条件,并观察其生物学特性。方法在无菌条件下抽取人脐血,用密度梯度离心的方法获得脐血单个核细胞,接种含10%胎牛血清的IMDM培养基中。对单个核细胞行贴壁培养后,进行细胞形态学观察,细胞生长、细胞周期分析及细胞凋亡检测,免疫细胞化学鉴定。结果采用Percoll(1.073g/ml)分离的脐血间充质干细胞(mesenchymal stem cells,MSCs)大小较为均匀,呈梭形或星形的上皮样细胞,传代培养后的细胞体积较大,成纤维样细胞逐渐增多。细胞生长曲线测定表明接种后第5d细胞进入指数增生期,至第9d进入平台期;流式细胞术证明G2+S期细胞为14.8%;免疫细胞化学染色结果表明42.0%细胞为CD45阳性。结论体外分离培养脐血间充质干细胞生长稳定,可作为组织工程的种子细胞。
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| 关 键 词： | 脐血 间充质干细胞 细胞培养 免疫细胞化学 |
The Biological Characteristics and Culturing Conditions of Human Umbilical Cord Mesenchymal Stem Cells |
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| ZHA La-ga-hu,MAI Xia,CHEN Xiao,CHEN Xiao-yi,CHEN Li. The Biological Characteristics and Culturing Conditions of Human Umbilical Cord Mesenchymal Stem Cells[J]. Progress of Anatomical Sciences, 2009, 15(2) |
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| Authors: | ZHA La-ga-hu MAI Xia CHEN Xiao CHEN Xiao-yi CHEN Li |
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| Affiliation: | 1.Department of Cell Biology;Medical College of Chinese People's Armed Police Forces;2.Department of Gynaecology and Obstetrics;Tianjin 300162 China |
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| Abstract: | Objective To optimize the isolation and culture conditions of mesenchymal stem cells(MSCs)in human umbilical cord in vitro,and explore their biological properties.Methods Human umbilical cord blood was collected in sterile condition,then the mononuclear cells were obtained by density gradient centrifugation and cultured in IMDM medium containing 10% fetal bovin serum.After the adherent mononuclear cells were obtained,the investigation and immunocytochemical identification of the cell cycles,cell growth,apop... |
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| Keywords: | Human umbilical cord mesenchymal stem cells cell culture immunocytochemistry human |
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