赤芍总甙对Bcl-2、c-myc基因表达影响及诱导细胞凋亡机制研究

目的：探讨赤芍总甙（TGC）诱导肿瘤细胞凋亡机制，为TGC的开发应用提供实验依据。方法：采用流式细胞仪测定细胞凋亡，SABC免疫组化法检测Bcl-2蛋白表达，原位杂交法测定c—myc mRNA表达，电镜观察凋亡小体。结果：TGC治疗组出现Ap峰GO-G1期细胞明显减少，S期细胞明显增多，与模型对照组比较有显著差异（P〈0．05）。实验组下调相关基因Bcl-2蛋白和c-myc mRNA表达水平。TGC组电镜可见细胞内膜结构完好，染色质边集，细胞核形成核带和核突，凋亡细胞数目较多，有新月形或花环状（凋亡小体形成）。结论：TGC诱导肿瘤细胞凋亡，其机制可能与下调Bcl-2和c-myc mRNA表达水平密切相关。

The influence of the total glucosides of radix paeoniae rubra on the expression of Bcl-2, c-myc gene and apoptosis mechanisms in tumor cell

Objective:To study TGC on tumor apopotosis and to apply the experimenting basis of TGC's developing and utilizing.Methods:The flow cytometer(FCM) was used for determination apoptosis.The protein expression of Bcl-2 and the mRNA expression of c-myc were evaluated by immunohistochemistry and in situ hybridization.Apoptosis small body were observed by EU.Results:The TGC group appear the Ap apex,the number of cell of G0-G1 period reduce obviously,the cells of S period increase obviously.Bcl-2 and the c-myc mRNA expression levels were decreased in TGC group.The structure of inner membrane was intacted with EU.Chromatin gathered side of cell,the nucleus turns into the nucleus band and nucleus project,there were many cells of the apoptosis and meniscus or the garland shape(the apoptosis corpuscle formatiol) in TGC group.Conclusion:The total glucosides of radix paeoniae induces the tumor cell apoptosising,its mechanism maybe closely related with adjusting expression levels of the Bcl-2 and the c-myc mRNA.