通心络胶囊对异丙肾上腺素致大鼠心肌损伤保护作用的实验研究

目的，探讨通心络胶囊对异丙肾上腺素致心肌损伤的保护作用，方法：于大鼠皮下注射异丙肾上腺素（85mg/kg)制备心肌损伤模型，34只大鼠随机分成生理盐水对照组，丙肾上腺素组及通心络胶囊干预组。HE染色及DNA末端标记法（TUNEL）分别检测心肌组织病理改变及心肌细胞凋亡的变化，透射电镜检测心肌细胞超微结构的变化。结果：生理盐水对照组无细胞变性与坏死，仅见极少凋亡阳性细胞；异丙肾上腺素组有明显心肌组织坏死。心肌凋亡细胞增多，电镜观察有凋亡细胞特征形态改变，通心络干预后心肌细胞坏死及凋亡均显著减轻。结论：异丙肾上腺素可致大鼠心肌组织坏死与凋亡，通心络胶囊可通过抑制心肌细胞坏死与凋亡而减轻心肌损伤。

Experimental Study of Protective Effect of Tongxinluo Capsule on Isoproterenol Induced Myocardial Injury in Rats

To study the protective effect of Tongxinluo capsule (TXLC) on myocardial injury induced by isoproternol. Methods: Myocardial injury was induced in 34 rats by subcutaneous injection of isoproterenol (85 mg/kg). The experimental animals were randomly divided into the control group, isoproterenol group and TXLC group. The histopathological change of myocardia was investigated by HE staining, the myocardial cell apoptosis were observed by TUNEL method and the ultrastructure of myocardial cell examined by electron microscope. Results: No cell degeneration and necrosis, only very few cells of apoptosis positive were found in the control group. While in the isoproterenol group, marked necrosis of myocardial tissue and increase of apoptosis cells were found, and characteristic changes of cell apoptosis were observed under electron microscope. After TXLC treatment, the myocardial necrosis and cell apoptosis were markedly alleviated. Conclusion: Isoproterenol could induce myocardial necrosis and apoptosis, TXLC could alleviate the myocardial injury through preventing myocardial cell necrosis and apoptosis.