玻璃化法处理同种异体动脉的移植

背景：体外血管预处理及保存方法有多种，经过学者们长期大量的研究观察，各种方法均得到了改进，但是仍然存在弊端。因此找到一种更有效的或者几种体外血管预处理保存方法的结合，还需要大量的研究实践。 目的：拟通过比较玻璃化法和传统冷冻法保存处理的同种异体血管移植后效果，找出一种更为实用且制作简便的血管处理方法。 方法：选用健康青紫兰兔96只，手术切除双侧股动脉，根据不同体外血管预处理方法将实验分为3组，即新鲜血管自体移植组、冷冻+辐照预处理血管同种异体移植组及玻璃化+辐照预处理血管同种异体移植组。血管移植后1，2，8，12周，每组取6只动物进行腹主动脉数字减影血管造影、扫描电子显微镜、组织病理学观察，观察移植血管通畅率、动脉瘤形成情况及组织形态学变化。 结果与结论：移植后12周，新鲜血管自体移植组血管的累计通畅率显著高于冷冻+辐照预处理组(P < 0.05)，玻璃化+辐照预处理组与其他两组比较，差异无显著性意义(P > 0.05)。组织病理学检查显示，玻璃化+辐照处理的同种异体血管内膜及中层平滑肌增生较冷冻+辐照预处理组轻，管腔狭窄不明显，炎症反应较轻。经玻璃化法+辐照保存的同种异体血管制作程序简单，移植血管通畅率高，组织反应轻，是一种比较理想的同种异体血管体外处理方法。

Vitrification treatment of allograft artery transplantation

BACKGROUND: Previous research has demonstrated that in vitro pre-treatment and conservation of blood vessels were improved remarkably; however, they remained still satisfactory. Therefore, to look for effective method still needs to be further studied. OBJECTIVE: To compare vitrification and traditional freezing to preserve the allograft vascular effects after transplantation, and to find a more practical and easy production of vascular treatment methods. METHODS: A total of 96 health bruising-blue rabbits, undergoing surgical resection of bilateral femoral artery, were divided into 3 groups: fresh vascular autograft (group A), irradiation pretreatment frozen vascular allograft (group B), and vitrification + irradiation pretreatment of vascular allograft (group C), according to different methods of in vitro vascular preconditioning experiment. At 1, 4, 8, and 12 weeks after transplantation, six experimental animals were taken from each group for abdominal aortic digital subtraction angiography, scanning electron microscopy, histopathology and other methods to observe the rate of aneurysm formation, morphological changes and organization of the vascular graft. RESULTS AND CONCLUSION: At 12 weeks after transplantation, the cumulative patency rate in the group A was significantly higher than that in the group B (P < 0.05); however, there was no significant difference between group C and group A as well as group B (P > 0.05). Histopathological examination revealed that allograft vascular smooth muscle proliferation in endometrium and middle layer of group C was slightly compared with group B, showing unclear lumen stenosis and light inflammatory reaction. The vitrification irradiation preservation of allograft blood vessels, making the procedure easy, high patency rate of grafts, tissue reaction to light, was an ideal way to deal with allogenic blood vessels in vitro.