小鼠骨髓间充质干细胞成骨分化过程中Caveolin-1的表达

目的:探讨小鼠骨髓间充质干细胞( bone marrow mesenchymal stem cells,BMSCs)向成骨细胞分化过程中小窝蛋白-1(Caveolin-1)的表达变化。方法:体外培养BMSCs细胞株,分为诱导成骨组和未诱导组,在培养的不同时期(3 d、7 d、10 d、14 d),采用碱性磷酸酶(AKP)活性测定、茜素红染色对BMSCs成骨分化进行鉴定,实时聚合酶链反应(real-time polymerase chain reaction RT-PCR)、蛋白质印迹法(Western Blot)检测各组细胞Caveolin-1 mRNA、蛋白的表达情况。结果:RT-PCR结果显示同一时间点诱导组Caveolin-1 mRNA表达量高于未诱导组,诱导组Caveolin-1 mRNA表达以时间依赖性的方式不断增高;Western Blot结果显示同一时间点诱导组Caveolin-1蛋白水平高于未诱导组,诱导组Caveolin-1蛋白水平以时间依赖性的方式增高。结论:本实验从mRNA、蛋白水平证实BMSCs表达Caveolin-1的量随着成骨分化的进行不断增加。Caveolin-1可能参与调控BMSCs成骨分化过程。

Expression of Caveolin-1 in Mouse Bone Marrow Mesenchymal Stem Cells during Osteogenic Differentiation

Objective: To investigate the expression of Caveolin-1 in mouse bone marrow mesenchymal stem cells (BMSCs) during osteoblast differentiation. Methods: The mouse BMSCs were cultured in vitro and divided into osteoblast-induced groups and non-induced group. In the different periods (3, 7, 10 and 14 days), the osteoblast differentiation was identified by alkaline phosphatase (AKP) activity and alizarin red staining and the expression of Caveolin-1 mRNA and protein in BMSCs were detected by real-time RT-PCR and Western blot. Results: RT-PCR results showed that osteoblast-induced groups had a higher expression level of Caveolin-1 than non-induced group at the same periods, and the Caveolin-1 mRNA expression was increased in a time-dependent effect in the osteoblast-induced groups. Western blot results showed that osteoblast-induced groups also had a higher expression level of Caveolin-1 than non-induced group at the same periods. The Caveolin-1 protein expression was increased in a time-dependent effect in osteoblast-induced groups. Conclusion: In this study, the Caveolin-1 expression at both mRNA and protein levels in BMSCs were increased with the osteogenic differentiation. Caveolin-1 may be regulated the BMSCs osteogenic differentiation process.