前列腺素E2对CD4+CD25+调节性T细胞免疫功能影响的实验研究

目的 探讨前列腺素E2对CD4+CD25+调节性T细胞免疫功能的影响。 方法 分离正常BALB/c小鼠脾脏CD4+CD25+ Treg，利用固相包被抗CD3和加入可溶性CD28辅助活化，予以PGE2刺激。根据刺激剂量不同，分成对照组、A (7 μmol/L)、B (14 μmol/L)、C (28 μmol/L)组，观察PGE2对Treg的增殖、IL-10、IL-2分泌和Foxp3表达的影响。 结果 PGE2刺激12 h，3组细胞较对照组增殖反应差异均无统计学意义(P＞0.05)；B、C组IL-2表达显著降低(P＜0.05)，而Foxp3表达明显升高(P＜0.05)；C组IL-10分泌升高(P＜0.05)；刺激24 h，B、C组细胞增殖反应较对照组显著增强(P＜0.05)；A、B、C组IL-2分泌均下降(P＜0.05)，而Foxp3表达均升高(P＜0.05)；C组IL-10分泌升高(P＜0.05)；刺激72 h，3组增殖反应、IL-10以及Foxp3表达均显著升高(P＜0.05)，而IL-2分泌下降(P＜0.05)。 结论 PGE2能直接增强CD4+CD25+ Treg的免疫抑制功能，从而可能进一步对效应T细胞免疫功能产生影响。 

Effect of prostaglandin E2 on the immune function of CD4^+CD25^+ regulatory T cells

Objective To explore the influence of prostaglandin E2 on the immune function of CD4+CD25+ regulatory T cells. Methods CD4+CD25+ Tregs isolated from the spleens of male BALB/C mice were seeded on 96-well cell culture plates coated with anti-CD3 and soluble anti-CD28,and cells were stimulated with prostaglandin E2.According to the different concentrations of PGE2 stimulation,the cells were divided into four groups:control,A(7 μmol/L),B(14 μmol/L),and C(28 μmol/L) groups.The proliferation of cells,IL-10,IL-2 and expression of Foxp3 in Tregs were determined at various intervals. Results After being stimulated with PGE2 for 12 h,the proliferation of cells in A,B,and C groups had no significant difference(P>0.05),and IL-2,Foxp3 expression in B,C groups were respectively down-regulated and up-regulated markedly(P<0.05),and IL-10 levels in C group was markedly up-regulated(P<0.05) compared with control group.For 24 h,the proliferation of cells in B,C groups were significantly increased(P<0.05),IL-2 levels in A,B,and C groups were significantly down-regulated(P<0.05),but Foxp3 expression was increased(P<0.05),IL-10 levels in C group was markedly up-regulated(P<0.05).For 72 h,the proliferation of cells,IL-10 and Foxp3 expression in A,B,and C groups was markedly up-regulated(P<0.05),but IL-2 levels in three groups were significantly down-regulated(P<0.05). Conclusion These data indicate that PGE2 stimulation can directly strengthen immunosuppression function of Treg in vitro,which may further exert some regulating effect on the immune function of effector T cells.