首页 | 本学科首页   官方微博 | 高级检索  
     

MMP-9基因敲除小鼠磨牙牙胚中釉原蛋白和成釉蛋白表达的研究
引用本文:李桐,袁国华,聂敏,杨国斌. MMP-9基因敲除小鼠磨牙牙胚中釉原蛋白和成釉蛋白表达的研究[J]. 口腔医学研究, 2016, 32(11): 1117. DOI: 10.13701/j.cnki.kqyxyj.2016.11.002
作者姓名:李桐  袁国华  聂敏  杨国斌
作者单位:武汉大学口腔医学院口腔生物医学工程教育部重点实验室 湖北 武汉 430079
基金项目:国家自然科学基金资助项目(编号:81371105,81570942)
摘    要:目的:检测MMP-9基因敲除小鼠(MMP-9-/-)和野生型小鼠磨牙牙胚中釉原蛋白和成釉蛋白的表达。方法:使用免疫组化方法检测出生后2 d(P2)、5 d(P5)、7 d(P7)野生型小鼠和MMP-9-/-小鼠下颌磨牙牙胚中釉原蛋白和成釉蛋白的表达分布。结果:在P2时,釉原蛋白主要表达在成熟的成釉细胞胞、及其相邻的成牙本质细胞内,敲除MMP-9后,釉原蛋白的表达可扩散至牙乳头间充质;在P5和P7时,釉原蛋白的表达分布在野生型小鼠和MMP-9-/-小鼠中无差异,但在MMP-9-/-小鼠中釉原蛋白在成釉细胞中的表达量明显增强。在P2、P5和P7时,成釉蛋白的表达分布和表达量在野生型小鼠和MMP-9-/-小鼠中均无差异。结论:MMP-9可能通过酶切釉原蛋白而影响其表达分布。

关 键 词:釉原蛋白  成釉蛋白  磨牙牙胚  基质金属蛋白酶9  
收稿时间:2016-06-27

Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice
LI Tong,YUAN Guo-hua,NIE Min,YANG Guo-bin. Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice[J]. Journal of Oral Science Research, 2016, 32(11): 1117. DOI: 10.13701/j.cnki.kqyxyj.2016.11.002
Authors:LI Tong  YUAN Guo-hua  NIE Min  YANG Guo-bin
Affiliation:School and Hospital of Stomatology, Key Lab for Oral Biomedical Engineering of Ministry of Education, Wuhan University, Wuhan 430079, China.
Abstract:Objective: To detect the expressions of Amelogenin and Ameloblastin in wild-type mice and MMP-9 gene knock-out mice (MMP-9-/-). Methods: The expression levels of Amelogenin and Ameloblastin in tooth germs were detected with immunohistochemistry in wild-type and MMP-9-/- mice at post-natal day 2 (P2), 5 (P5), and 7 (P7). Results: At P2, Amelogenin was mainly expressed in matured ameloblasts and the underlying odontoblasts. However, in MMP-9-/- mice, the expression of amelogenin was expanded to the dental mesenchyme. No difference was found in the expression pattern of Amelogenin between wild-type mice and MMP-9-/- mice at P5 and P7. However, the expression level of Amelogenin in ameloblasts was highly increased in MMP-9-/- mice compared with that in wild-type mice. No obvious differences were detected in the expression pattern or expression level of Ameloblastin between wild-type mice and MMP-9-/- mice at P2, P5, and P7. Conclusion: MMP-9 might be able to degrade Amelogenin, and further influence the expression pattern of Amelogenin.
Keywords:Amelogenin  Ameloblastin  Molar germ  Matrix  Metalloproteinase-9  
点击此处可从《口腔医学研究》浏览原始摘要信息
点击此处可从《口腔医学研究》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号