2012-2014年湖南省感染性腹泻哨点医院儿童诺如病毒感染及基因型别分析

目的 了解湖南省感染性腹泻哨点医院儿童诺如病毒的感染状况及基因型别。 方法 2012年1月-2014年12月采集湖南省哨点医院腹泻患儿的粪便标本,应用诺如病毒特异性引物进行扩增,选择扩增阳性产物进行基因测序和进化分析。 结果 936份粪便标本RT-PCR检测诺如病毒核酸,阳性100份,阳性率10.68%。对40份诺如病毒核酸阳性标本进行基因序列测定与分析,38份为GⅡ型,其中31份为GⅡ.4型。在31株GⅡ.4型中,Sydney 2012和GII.4 Den Haag 2006b各栓出14株。 结论 2012-2014年湖南省哨点医院儿童腹泻病例中诺如病毒的感染率较高,GⅡ.4是优势株,其中GⅡ.4 sydney 2012和Den Haag 2006b是主要型别。

Infection and genotypes of norovirus among children with acute diarrhea in sentinel hospitals in Hunan Province, 2012 -2014

Objective To investigate the infection situation of norovirus and its genotypes among children with acute diarrheain sentinel hospitals in Hunan Province. Methods Fecal specimens were collected from 936 chirdren with acute diarrhea in sentinel hospitals in Hunan Province from January 2012 to December 2014. RT-PCR was used to detect norovirus and obtain positive samples. Phylogenetic analysis and genotyping were performed. Results Totally, 100 out of 936 samples were postive for norovirus, with the positive rate of 10.68%. 38 out of 40 sequenced strains were GII, among which 31 isolates were GII.4 variants. GII.4 Sydney 2012 and GII.4 Den Haag 2006b were respectively detected in 14 isolates. Conclusions Norovirus has a high detection rate in children with acute diarrhea in sentinel hospitals in Hunan Province, 2012-2014. GII.4 variant is the predominant strain, and GII.4 Sydney 2012 and GII.4 Den Haag 2006b are the predominant genotypes.