纯钛超声微弧氧化-壳聚糖/海藻酸钠自组装种植体的动物实验研究

目的: 采用层层自组装技术(Layer-by-Layer self-assembly technique)在纯钛(TA2)微弧氧化表面制备壳聚糖(CS)/海藻酸钠(ALG)复合涂层,研究对骨结合的影响。方法: 选取24只新西兰大白兔建立双侧下颌骨种植模型,分别植入A、B、C组,TA2-MAO为A组,TA2-MAO-NAOH-CS为B组,TA2-MAO-NAOH-CS-ALG为C组。术后分别于2、4、6周取材,采用锥形束CT(cone beam,CBCT)和种植体骨扭出力测试的方法,观察种植体植入后骨结合的情况。选扭出6周的种植体做扫描电镜(SEM), 观察种植体涂层表面形貌。结果: CBCT观察:在2、4、6周,C组骨缺损区的愈合情况明显优于A、B组,B组骨缺损区的愈合情况稍好于A组,在6周时,观察到C组骨愈合良好,种植体骨界面无明显阴影区域,骨密度与正常骨密度一致。种植体骨扭出力测试中观察:在第2周时,A、B、C组的扭出力差异不明显,第4周时,B、C组扭力值高于A组,第6周时,C组的扭出力明显大于A、B组。在6周时,扫描电镜可以观察到c组对骨结合有促进作用。结论: TA2-MAO-NAOH-CS涂层和TA2-MAO-NAOH-CS-ALG涂层对骨结合都有促进作用,而TA2-MAO-NAOH-CS-ALG复合涂层对骨结合效果更好,双侧建立动物模型的方法可行。

Animal Study on Pure Titanium Implant with Micro-arc Oxidation and Self-assembly GTS/ALG Coating.

Objective: To evaluate the effects of layer-by-layer self-assembly technique and micro-arc oxidation surface on bone integration of pure titanium implant. Methods: Twenty-four New Zealand white rabbits were selected to construct animal models. Two, 4 and 6 weeks after operation, CBCT and implant bone torsion output test were performed to observe osseointegration of implants. Six weeks after operation, scanning electron microscopy (SEM) was used to observe the surface morphology of implants. Results: CBCT results showed that at post-operative 2, 4 and 6 weeks, the healing of bone defect was good in the sequence of group C, group B and group A. At post-operative 6 weeks, there were no obvious dash areas on implant bone interface, and the density was similar to normal bone. Implant bone torsion output test results showed that there were no obvious differences at post-operative 2 weeks in torsion. At post-operative 4 weeks, the torsion was higher in group B and C compared to group A. At post-operative 6 weeks, the torsion was the highest in group C compared to group A and B. Moreover, at post-operative 6 weeks, osseointegration was significantly increased in group C. Conclusion: TA2-MAO-NAOH-CS coating and TA2-MAO-NAOH-CS-ALG coating could promote the bone integration, and the effects of TA2-MAO-NAOH-CS-ALG coating were better.