Bio-oss骨粉对人胎盘来源间充质干细胞体外成骨活性影响的研究

目的: 研究人胎盘源间充质干细胞(HPMSCs)与Bio-oss骨粉结合后的增殖分化情况,探讨以人胎盘源干细胞(HPMSCs)作为种子细胞,Bio-oss骨粉作为支架材料联合构建组织工程化生物衍生骨的可能性。方法: 无菌条件下分离培养人胎盘源间充质干细胞(HPMSCs)并检测其分化能力。将细胞分为单独培养组与复合Bio-oss骨粉培养组。观察细胞与支架材料结合情况并在细胞形态特点、增殖分化能力方面将两组细胞进行比较。结果: 人胎盘源间充质干细胞与Bio-oss骨粉复合培养后,细胞形态、增殖分化能力与对照组人胎盘源间充质干细胞无明显差异。结论: 以人胎盘源干细胞(HPMSCs)为种子细胞、Bio-oss骨粉为支架复合培养构建组织工程化衍生骨是可行的。

Effects of Bio-oss on Osteogenesis of Human Placenta-derived Mesenchymal Stem Cells in Vitro.

Objective: To study the proliferation and differentiation of human placenta-derived mesenchymal stem cells (HPMSCs) combined with Bio-oss powder,and investigate the feasibility of constructing tissue engineered bone by HPMSCs as seed cells and the Bio-oss powder as scaffold material. Methods: Human placenta-derived mesenchymal stem cells were dissociated and cultured under sterile conditions and detected for the ability of differentiation. They were divided into two groups, the group of combination of cells and Bio-oss as scaffold material and the group of cells alone. Cell morphology, proliferation and differentiation in the two groups were observed and compared. Results: There was no significant difference between two groups on the cell morphology, proliferation and differentiation ability. Conclusion: It is feasible to utilize HPMSCs as seed cells and Bio-oss as scaffolds to construct tissue engineered bone.