川芎嗪对脑缺血再灌注时细胞凋亡的影响

目的：探讨川芎嗪对脑缺血再灌注时细胞凋亡的影响。方法：对实验大鼠于脑缺血前应用川芭嗪进行干预，然后采用免疫组化法检测脑缺血再灌注不同时间内凋亡相关基因ｃ－ｂｃｌ－２蛋白表达的变化。结果：川芎嗪组大鼠脑缺血后的梗死体积显著减小，川芎嗪组脑缺血再灌注时皮层和基底节区ｃ－ｆｏｓ的表达明显下降，而ｂｃｌ－２的表达明显增高。结论 川芎嗪可抑制脑缺血再灌注时细胞凋亡的发生，具有神经保护作用。

The effects of ligustrazini on apoptosis after focal cerebral ischemia followed by reperfusion

ObjectiveTo investigate the effect of Ligustrazini(LZ)on apoptosis after focal cerebral ischemia followed by reperfusion.Methods:Rats were pretreated with LZ prior to MCAO,changes of protein expression of cfos and bcl2 in rats after ischemia and reperfusion were observed by immunohistochemical method.Results:The infarct volume in LZ group was significantly smaller than that in the corresponding regions in the control group.In LZ group ,the protine expression of cfos decreased significantly after ischeima/reperfusion both in the ischemic cortex and in the ischemic basal ganglia ,but the protein expression of bcl2 increased significantly after ischemia/reperfusion both in the ischemic ocrtex and in the ischemic basal ganglia.Conclusions:LZ may inhibit the development of apoptosis in rats after ischemia and reperfusion and have the neuroprotective effect against ischemia.