| 荧光原位杂交检测人类免疫缺陷病毒-1 DNA对感染者外周血淋巴细胞染色体的整合 |
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| 引用本文： | 王典,李练兵,侯志伟,谢庆东,马明福,康祥锦,黄天华.荧光原位杂交检测人类免疫缺陷病毒-1 DNA对感染者外周血淋巴细胞染色体的整合[J].汕头大学医学院学报,2010,23(4):196-197,201,F0002. |
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| 作者姓名： | 王典 李练兵 侯志伟 谢庆东 马明福 康祥锦 黄天华 |
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| 作者单位： | 汕头大学医学院生殖医学研究中心;汕头大学医学院法医学教研室;重庆市出生缺陷与生殖健康市级重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的：探讨荧光原位杂交（FISH）检测人类免疫缺陷病毒（HIV-1）前病毒DNA对外周血淋巴细胞（PBL）染色体整合的可行性与效率。方法：收集3例HIV-1感染者,常规制备其PBL染色体,采用FISH方法,以生物素标记的HIV-1gag及HIV-1pol的cDNA作为探针,检测HIV前病毒在其中的整合。结果：观察了172套来源于3位感染者PBL的染色体,6套有阳性荧光信号。结论：FISH可用于检测HIV前病毒DNA对患者PBL染色体整合,且是一种灵敏、可靠的方法。
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| 关 键 词： | 荧光原位杂交 人类免疫缺陷病毒 整合 外周血淋巴细胞染色体 |
Detection of the Integration of Human Immunodeficiency Virus-1 Provirus DNA into the Chromosome of Peripheral Blood Lymphocyte in Infected Patients by Fluorescence in Situ Hybridization |
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| WANG Dian,LI Lian-bing,HOU Zhi-wei,XIE Qing-dong,MA Ming-fu,KANG Xiang-jin,HUANG Tian-hua.Detection of the Integration of Human Immunodeficiency Virus-1 Provirus DNA into the Chromosome of Peripheral Blood Lymphocyte in Infected Patients by Fluorescence in Situ Hybridization[J].Journal of Shantou University Medical College,2010,23(4):196-197,201,F0002. |
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| Authors: | WANG Dian LI Lian-bing HOU Zhi-wei XIE Qing-dong MA Ming-fu KANG Xiang-jin HUANG Tian-hua |
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| Institution: | WANG Dian1,LI Lian-bing2,HOU Zhi-wei2,XIE Qing-dong1,MA Ming-fu2,KANG Xiang-jin1,HUANG Tian-hua1(1 Research Center for Reproductive Medicine,Shantou University Medical College,Shantou 515041 China,2 Chongqing Key Laboratory of Birth Defects and Reproductive Health,Chongqing 400020,3 Department of Forensic Medicine,Shantou 515041,China) |
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| Abstract: | Objective:To study the possibility and efficiency of fluorescence in situ hybridization(FISH)to detect the integration of human immunodeficiency virus-1(HIV-1)provirus DNA into the chromosome of peripheral blood lymphocyte(PBL)in infected patients.Methods:Three HIV infected patients were enrolled.PBL chromosomes of them were prepared routinely,and then FISH was performed to detect the integration of HIV-1 provirus DNA into these chromosomes.Biotinylated HIV-1 gag and HIV-1 pol were used as probes in FISH.Re... |
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| Keywords: | fluorescence in situ hybridization human immunodeficiency virus-1 integration chromosome of peripheral blood lymphocyte |
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