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Activation of guinea pig eosinophil respiratory burst by leukotriene B4: role of protein kinase C
Authors:KF Rabe  MA Giembycz  G. Dent  and PJ Barnes
Affiliation:Department of Thoracic Medicine, National Heart and Lung Institute, London, UK.
Abstract:Leukotriene B4 (LTB4) and the protein kinase C activator, 4-beta-phorbol dibutyrate (PDBu), both induced a pronounced and concentration-dependent stimulation of hydrogen peroxide (H2O2) generation by purified guinea pig peritoneal eosinophils in the concentration range 1 nM-1 microM. The LTB4 response was inhibited competitively by the specific LTB4 receptor antagonist, U-75302, with a KB of 25 nM, while the concentration-response curves for both stimuli were shifted rightwards (3.8-fold and 2.8-fold for LTB4 and PDBu, respectively) by the competitive protein kinase C inhibitor, 1-O-hexadecyl-2-O-methylglycerol at a concentration of 300 microM. LTB4 appears, therefore, to induce respiratory burst in eosinophils via a receptor-mediated mechanism involving protein kinase C.
Keywords:leukotriene B4    eosinophils    respiratory burst    protein kinase C    protein kinase C inhibitors    LTB4 antagonists    U-75302
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