电针、手针“足三里”对慢性关节炎大鼠血清炎性细胞因子含量的影响

目的:观察不同针刺方法对慢性佐剂性关节炎大鼠血清中致炎类因子白介素1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和抗炎类因子白介素2(IL-2)、白介素4(IL-4)及白介素10(IL-10)含量的影响,比较不同针刺方法的效应。方法:将SD大鼠随机分为正常组、模型组、电针单穴组、电针双穴组和手针单穴组,每组12只。大鼠右后肢足跖皮内注射0.1mL弗氏完全佐剂制造佐剂性关节炎模型。针刺各组于造模第11天起取"足三里"穴进行治疗,每次30min,每周治疗2次,共4周。以酶联免疫法检测大鼠血清中IL-1β、TNF-α、IL-2、IL-4及IL-10含量。结果:与正常组相比,模型组大鼠血清中IL-1β、TNF-α、IL-4及IL-10的含量均显著升高(P<0.001)。与模型组相比,各治疗组大鼠血清中致炎类因子IL-1β和TNF-α含量明显降低(P<0.001,P<0.05),抗炎类因子IL-2含量明显升高(P<0.05);各治疗组大鼠血清中的IL-4、IL-10水平在炎性反应升高后向正常方向回落(P<0.001,P<0.05)。电针单穴组、电针双穴组和手针单穴组之间各指标的差异无统计学意义(P>0.05)。结论:三种针刺方法即电针单穴、电针双穴和手针单穴均可提高慢性佐剂性关节炎大鼠的抗炎修复功能,且三种针刺方法治疗效应相当,其机制可能是通过对机体炎性细胞因子的良性诱导,改变相关炎性细胞因子的合成与释放。

Effects of Electroacupuncture and Manual Acupuncture Interventions on Contents of Serum Inflammatory Cytokines in Rats with Chronic Adjuvant Arthritis

Objective To observe the effect of electroacupuncture(EA) or manual acupuncture of unilateral or bilateral "Zusanli"(ST 36) on the contents of serum inflammatory factors interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α),and anti-inflammatory factors interleukin 2(IL-2),interleukin 4(IL-4) and interleukin 10(IL-10),so as to reveal their mechanism underlying improvement of chronic arthritis.Methods Sixty SD rats were randomly divided into normal control,model,electroacupuncture(EA)-left(L) Zusanli(ST 36),EA-bilateral(Bi)-ST 36,and manual acupuncture(MA)-L-ST 36 groups(12 rats/group).Arthritis model was established by subcutaneous injection of complete Freund’s adjuvant(0.1 mL)into the hindpaw.EA(2 Hz/100 Hz,1-3 mA,pulse duration: 0.6 ms/0.2 ms) or manual needle stimulation was applied to the left or bilateral "Zusanli"(ST 36) for 30 min,twice every week for 4 weeks.Enzyme linked immunosorbent assay(ELISA) was used to detect the contents of serum IL-1β,TNF-α,IL-2,IL-4 and IL-10,respectively.Results Compared with the normal control group,the contents of serum inflammatory factors IL-1β and TNF-α,anti-inflammatory factors IL-4 and IL-10 were increased significantly in the model group(P<0.001).In comparison with the model group,serum IL-1β,TNF-α,IL-4 and IL-10 contents were down-regulated considerably in the EA-L-ST 36,EA-Bi-ST 36 and MA-L-ST 36 groups(P<0.001,P<0.005).Serum IL-2 content had no apparent changes after modeling in comparison with the normal group(P>0.05).While serum IL-2 contents were markedly higher in the EA-L-ST 36,EA-Bi-ST 36 and MA-L-ST 36 groups than in the model group(P<0.05).No significant differences were found among the EA-L-ST 36,EA-Bi-ST 36 and MA-L-ST 36 groups in serum IL-1β,TNF-α,IL-2,IL-4 and IL-10 contents(P>0.05).Conclusion EA or manual acupuncture stimulation of single or bilateral ST 36 can effectively down-regulate serum inflammatory factors IL-1β and TNF-α levels,and upregulate anti-inflammatory factor IL-2 level in chronic arthritis rats,which may contribute to their effects in relieving chronic arthritis.