Gene therapy with antisense c-myc adenovirus for human gastric carcino- ma cell line in vitro and for implanted carcinoma in nude mice |
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| Authors: | Chen Jieping Lin Cheng Xu Caipu Zhang Xueyan Fu Ming Deng Youping Wei Yue Wu Ming |
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| Institution: | Chen Jieping, Lin Cheng ,Xu Caipu, Zhang Xueyan , Fu Ming, Deng Youping, Wei Yue, Wu Ming Department of Gastroenterology,Southwest Hospital,Third Military Medical University,Chongqing 400038, Cancer Institute,Chinese Academy of Medical Science,Bei |
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| Abstract: | Objective:To study the effects of recombinant antisense c-myc adenovirus (rAS-c-myc-Ad) on SGG 7901 human gastric carcinoma cell line in for and in nude mice. Methods:The effects of rAS-c-myc-Ad and LacZ-Ad on SGG 7901 gastric carcinoma cells were observed with X-galstaining, MTT, DNA gradient degradation test, TUNEL, flow cytometry, PCR and western blot. The therapeutic effects of rAS-c-myc-Ad on the implanted ax 7901 cells in nude mice were also ob served.Results: rAS-c-myc-Ad significantly inhibited the growth of SGG 7901 cells and induced their apoptosis. After the treatment of rAS-c-myc-Ad, the prolifetion rate of the cells was decreased by 44' l% in de and SGC 7901 cells failed to form caxcinoma ther they were implanted into nude mice. Injection of rAS-c-myc-Ad into the carcinoma subcutaneously implanted to the nude mice significantly inhibited the growth of the implanted carcinoma with an inhibition rate of 68. 9%. Conclusion: rAS-c- myc- Ad significantly inhibits the growth of SGG 7901 human gastric carcinoma cells in vitro and in nude mice |
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| Keywords: | gastric carcinoma gene therapy c-myc adenovirus nude mouse |
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