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| 过钒酸钠对无EPO状态下BET-2细胞线粒体膜电位与细胞凋亡的影响 | |
| 引用本文： | 杨龙,许祥裕,董波,从玉文,陈家佩. 过钒酸钠对无EPO状态下BET-2细胞线粒体膜电位与细胞凋亡的影响[J]. 微量元素与健康研究, 2009, 26(1) |
| 作者姓名： | 杨龙 许祥裕 董波 从玉文 陈家佩 |
| 作者单位： | 1. 南京军区军事医学研究所博士后科研工作站,南京,210002 2. 军事医学科学院放射与辐射医学研究所,北京,100850 |
| 基金项目： | 国家自然科学基金，江苏省人事厅博士后专项基金 |
| 摘 要： | 目的:观察酪氨酸磷酸酶(protein tyrosine phosphotases,PTP)抑制剂过钒酸钠(sodium Pervanadate,Per)对去除EPO状态下BET-2细胞存活、增殖、线粒体膜电位改变与细胞凋亡的影响。方法:应用MTT法、流式细胞仪等方法观察Per对EPO依赖的小鼠源性髓系白血病细胞株BET-2细胞在无EPO状态下细胞存活、增殖、细胞线粒体膜电位改变、细胞凋亡的影响。结果:BET-2细胞对EPO有良好的依赖性,在无EPO状态下BET-2细胞增殖停滞、细胞线粒体膜电位下降,并出现细胞凋亡;适当浓度Per(1~31.25μmol/L)能促进无EPO状态下BET-2细胞存活、增殖,维持细胞线粒体膜电位,减少细胞凋亡。结论:通过Per等调控PTP活性可能促进EPO依赖的BET-2细胞在EPO缺乏状态下的存活、增殖。 |
| 关 键 词： | BET-2细胞 EPO 线粒体膜电位 细胞凋亡 |
Effects of Pervanadate on the changes of mitochondrial membrane potentialand apoptosis in BET-2 cells in non-EPO Condition |
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| YANG Long,XU Xiang-yu,Dong Bo,et al. Effects of Pervanadate on the changes of mitochondrial membrane potentialand apoptosis in BET-2 cells in non-EPO Condition[J]. Studies of Trace Elements and Health, 2009, 26(1) | |
| Authors: | YANG Long XU Xiang-yu Dong Bo et al |
| Affiliation: | YANG Long1,XU Xiang-yu1,Dong Bo2,et al |
| Abstract: | Objective: To study the effects of Pervanadate(Per) on the changes of growth-responsiveness,mitochondrial membrane potential and apoptosis in BET-2 cells in non-EPO condition.Methods:The BET-2 cells were divided into PTP-specific inhibitor Per-treated group and non-Per group(served as a control) and then incubated under non-EPO condition.The changes of growth-responsiveness in BET-2 cells were observed by MTT methods,the fluctuation of mitochondrial membrane potential and apoptosis were measured by FCM.Resu... |
| Keywords: | BET-2 cell EPO growth-responsiveness Pervanadate(Per) |
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