| 含CD基因的腺病毒载体制备及体外抑瘤效果观察 |
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| 引用本文： | 戴观荣,高军,王元和,崔龙. 含CD基因的腺病毒载体制备及体外抑瘤效果观察[J]. 第二军医大学学报, 2001, 22(1): 47-49 |
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| 作者姓名： | 戴观荣 高军 王元和 崔龙 |
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| 作者单位： | 长征医院普通外科;海军医学研究所;第二军医大学长海医院普通外科, |
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| 基金项目： | 国家自然科学基金资助项目!(396 0 0 172 ) |
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| 摘 要： | 目的：制备含胞嘧啶转氨酶（CD）基因的重组腺病毒（Ad-CD)并进行体外肿瘤的自杀基因治疗。方法：构建含CD或LacZ基因的重组腺病毒载体。在293细胞内重组包装后,体外感染小鼠结肠癌细胞株C26,并给予不同浓度的前药氟胞嘧啶（5-FC）进行肿瘤杀伤效果观察。结果：重组腺病毒载体构建成功。用制备的Ad-CD液感染C26细胞,并予以不同浓度5-FC后,肿瘤细胞生长受不同程度抑制,而对照组细胞和原位转LacZ基因的C26细胞的生长未出现明显的变化。结论：应用腺病毒载体转CD基因进行体外肿瘤治疗效果明显,为自杀基因应用于体内结肠癌的基因治疗研究奠定基础。
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| 关 键 词： | 胞嘧啶转氨酶基因 腺病毒 基因疗法 |
| 文章编号： | 0258-879X(2001)01-0047-03 |
| 修稿时间： | 2000-06-10 |
Preparation of an adenovirus expressing cytosine deaminase and observat ion on its tumor-killing effect in vitro |
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| DAI Guan-rong,WANG Yuan-he,GAO Jun,Cui Long. Preparation of an adenovirus expressing cytosine deaminase and observat ion on its tumor-killing effect in vitro[J]. Former Academic Journal of Second Military Medical University, 2001, 22(1): 47-49 |
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| Authors: | DAI Guan-rong WANG Yuan-he GAO Jun Cui Long |
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| Abstract: | Objective: To prepare an adenovirus, Ad CD, expressing bacterial cytosine deaminase(CD),and use it for tumor suicide gene therapy. Methods: We constructed a recombined adenoviral vector carrying CD or LacZ. 293 packaging cell was transfected by the newly constructed plasmid and generated the infectious virus. Then the adenovirus was used to infect C26 cell in vitro . Results: The growth of Ad CD infected cell was obviously surpressed after administration of 5 FC. Conclusion: CD gene carried by adenoviral vector is effective for tumor cell suicide gene therapy in vitro . |
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| Keywords: | cytosine deaminase gene adenovirus vector gene therapy |
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