BBB评分评估脊髓损伤大鼠后肢运动功能的探讨

目的：探讨大鼠脊髓损伤后和修复中如何评估人鼠后肢运动的BBB评分。方法：对4组大鼠分别行T10脊髓背侧半切断(A组)、T10脊髓全切断(B组)、T10脊髓节段全切除(C组)、T10以下脊髓全切除(D组)，制成不同损伤程度的大鼠脊髓损伤模型，对所有动物的后肢运动功能进行BBB评分和脊髓组织学观察。结果：A组大鼠BBB评分存损伤后5崩达到20分或21分，B组和C组大鼠存术后2周以后BBB评分维持在8分．D组大鼠BBB评分维持在0。B组和C组大鼠脊髓顺行追踪显示脊髓损伤区和尾侧无追踪剂分布．连续矢状冰冻切片抗神经丝(NF)染色未见连续NF通过损伤区，结论：大鼠脊髓损伤模型的后肢运动功能BBB评分如果在8分以下，就需要慎重评价，这种运动有可能完全是或包括有自发的后肢运动。

Study on the BBB scale of hindlimb movements recovery in rats of spinal cord injury

Objective:To understand how to exactly evaluate the BBB scales of hindlimb movements in spinal cord injury of rats.Method:Four groups of spinal cord injury rat models including T10 dorsal hemisection,T10 transection,T10 segment resection,segments caudal to T10 resection were adopted,histological examinations of spinal cord and BBB scales of hindlimb movements were observed and the relationship between hindlimb movements and BBB scales was evaluated.Result:The BBB scales reached 20 or 21 in animals of T10 dorsal hemisection and maintained 0 in animals of segments caudal to T10 resection.The BBB scales maintained 8 in all the animals of T10 hemisection group or T10 resection group two weeks after operation.Anterograde tracing by BDA showed no BDA presented in the lesion area and the caudal in T10 hemisection group or T10 resection group.Anti-NF staining showed no continuous NF traversed the lesion area.Conclusion:It should be prudently evaluated in the hindlimb movements of BBB scales less than 8 in spinal cord injury of rats,since it is completely or it includes the spontaneous recovery at least.