应用cDNA微阵列技术检测子宫内膜癌组织中基因表达图谱的变化

目的：通过研究正常子宫内膜和子宫内膜癌组织基因表达的变化，来探讨子宫内膜癌发的生机制，方法：应用cDNA微阵列技术（含588基因），对30例子宫内膜癌患者的癌组织及同一患者的正常子宫内膜组织进行分析，由两种组织中分别提取总RNA后，制备探针，与cDNA微阵列膜进行杂交，杂交结果经计算机软件分析，采用免疫组织化学的方法对结果进行鉴定，结果：（1）与细胞死亡和增殖有关的基因－IRP，Wnt5-5A及癌胚抗原前体在癌组织中表达的明显增高；（2）与细胞粘附，浸润有关的基因－聚集蛋白降糖核蛋白前体在癌组织中的表达明显增高，（3）与浸润有关的调节因子基因－CD147，TIMP－2基因在癌组织中的表达异常增高，结论：用cDNA微阵列可同时检测两种不同组织中多个基因的差异表达，CD147可能与子宫内膜癌的发生与浸润有关。

Monitoring gene expression profile changes in endometrial cancer using cDNA microarray technology

Objective To study the difference in gene expression between normal endometrial tissue and endometrial cancer tissue so as to investigate the mechanism of pathogenesis of endometrial cancer. Methods The tissues from 30 patients with endometrial cancer and normal endometrial tissues from the same patients were analyzed by cDNA microarray technology (including 588 genes). General RNA was extracted from these tissues and probes were made to be hybridized with cDNA array membrane. The CD147 expressions in normal endometrial tissue and in endometrial cancer tissue were identified by immunohistochemistry. Results The endometrial cancer associated genes -- IRP, Wnt 5A, 5T4 oncofetal antigen precursor, aggrecan core protein precursor, CD147, TIMP 2, were expressed highly. Conclusion cDNA microarray technology can simultaneously monitor the different expressions of genes from two different tissues. CD147 might be useful in assessing the progression and infiltration of endometrial cancer.