COX-2抑制剂对慢性铝过负荷大鼠海马5-脂氧酶表达的影响

目的探讨COX-2抑制剂美洛昔康对慢性铝过负荷大鼠海马5-LO表达的影响。方法葡萄糖酸铝灌胃给予Wistar大鼠,1次/d,每周5 d,连续20 w,建立慢性铝过负荷致神经元退变大鼠模型。美洛昔康(1和3 mg/kg),在每次给予铝盐后30 min灌胃给予。RT-PCR检测大鼠海马5-LO mRNA的表达变化,Western印迹方法检测5-LO蛋白表达情况。结果慢性铝过负荷致大鼠海马5-LO mRNA和蛋白表达明显增加。美洛昔康能明显阻遏慢性铝过负荷诱导的大鼠海马5-LO mRNA和蛋白表达的增加。结论 COX-2抑制剂明显下调慢性铝过负荷大鼠海马5-LO表达。

Effect of COX-2 inhibitor on 5-LO expression of hippocampus in chronic aluminum overload-induced neurodegenerative rats

Objective To investigate the effect of meloxicam on 5-LO expression of hippocampus in chronic aluminum overload induced neurodegeneration rats.Methods Neurodegeneration model of Wistar rats were established by intragastric administration of aluminum gluconate(Al3+200 mg/kg),once a day,5 d/week,for 20 weeks.Meloxicam(1 and 3 mg/kg) was intragastrically administered 30 min after each aluminum administration.The expression of 5-LO mRNA and protein in hippocampus were detected by RT-PCR and Western blot respectively.Results Expression of 5-LO mRNA and protein in hippocampus obviously were increased.Meloxicam obviously protected rats from learning and memory function impairment and neuron death,significantly inhibited increase of the expression of 5-LO mRNA and protein induced by chronic aluminum overload.Conclusions Meloxicam can downregulate the 5-LO expression of hippocampus in chronic aluminum overload induced neurodegenerative rats.