郑州市主要生活饮用水源微囊藻细胞毒素特征分析

目的分析郑州市主要生活饮用水源微囊藻细胞毒素特征。方法以西流湖和黄河花园口段某调蓄池作为调查现场,采用96孔板结和极限稀释法对采集藻细胞进行分离纯化;应用全细胞PCR方法检测所分离微囊藻细胞株藻青蛋白基因中间序列(PC-IGS)和微囊藻毒素多肽合成酶基因B(mcyB),并对提取毒素应用固相萃取(SPE)高效液相色谱(HPLC)法进行检测。结果自西流湖分离的XLH细胞株和黄河花园口调蓄池分离的2株微囊藻细胞BM1、BM2,PC-IGS、mcyB基因扩增均为阳性;mcyB基因序列测定结果与Genbank中报道的mcy基因同源性达99%;HPLC检测3株藻细胞所含毒素异构体主要为MC-LR,占毒素总量的质量百分比分别为97.9%、98.6%和99.3%。结论自郑州市主要生活饮用水源分离的3株微囊藻细胞均为产毒株,产生毒素异构体主要为毒性较大的MC-LR。

Characteristic analysis on the toxin of microcystis in life water source of Zhengzhou City

Objective To analyze the toxic characteristic of microcystis in a life water source. Methods Microcystis in Huayuankou conservation pool and Xiliu lake were isolated by 96-microbiological assay and supreme dilution methods. Phycocyanin intergenic spacer region (PC-IGS) and microcystin synthetase gene B(McyB) were detected by whole-cell PCR method, And toxicities of microcystis were identified by solid pole of extraction(SPE) and high performance liquid chromatography(HPLC) method. Results The microcystis of the water source were cyanobacteria which could contain PC-IGS and McyB expressing positively, Comparition with reported mcyB of Microcystis aeruginosa in Genbank, the homology of gene sequence was 99%. The main toxic isomers of the three microcystis were MC-LR, whose proportions of the whole toxin were 97.9%,98.6% and 99.3%,respectively. Conclusion The microcystis of the main life water source maybe poisonous and the main isomer maybe MC-LR.