白藜芦醇对鼠纤维肉瘤S180细胞周期影响及其机制研究

目的:研究白藜芦醇对鼠纤维肉瘤S180细胞的抗肿瘤作用,并探讨其分子机制.方法:用MTT法检测白藜芦醇对鼠纤维肉瘤细胞系S180增殖的影响;PI单标流式细胞术检测白藜芦醇对S180细胞周期分布的影响;Western blot方法检测白藜芦醇对S180细胞 cyclinD1,P21Cip1蛋白表达的影响.结果:白藜芦醇对S180细胞增殖呈双相作用:低浓度促进细胞增殖;高浓度抑制细胞增殖,其抑制作用呈时效和量效关系.白藜芦醇治疗组S180细胞周期发生变化,细胞周期被阻滞于 G0/G1期.白藜芦醇以时间依赖方式下调周期蛋白 cyclinD1 的表达.上调白藜芦醇P21Cip1蛋白表达.结论:白藜芦醇低浓度促进S180细胞增殖,高浓度抑制细胞增殖.白藜芦醇可通过凋节细胞周期蛋白 cyclinD1,p21Cip1的表达调控细胞周期进程,抑制细胞增殖.

Effects of Resveratrol on cell cycle regulatory processes of mice fibrosarcoma S180 cells and their mechanisms

Objective:To investigate the anticancer activities of Resveratrol on mice fibrosarcoma S180 cells and their molecular mechanisms involved. Methods:The effects of Resveratrol on the growth of mice fibrosarcoma cell lines S180 were studied by MTT assay. The effect of Resveratrol on the cell cycle phase distribution of S180 cells were analyzed using flow cytometry by a propidium iodide method. The effect of Resveratrol on the expression of cyclinD1 and P21Cip1 protein was studied by Western blot analysis. Results:Resveratrol had a dual effect on the growth of S180 cells:It increased the growth of S180 cells at low concentrations,and it inhibited the proliferation of S180 cells in a time-and dose-dependent manner at high concentrations. Flow cytometric analyses showed that S180 cells treated with Resveratrol were accumulated in G0/G1 phase in the cell cycle,the ratio of cells in G2/M phase was decreased at 24 h treatment. The expression of cyclinD1 protein was decreased in a time-dependent manner in S180 cells treated with Resveratrol,while P21Cip1 protein was increased. Conclusion:Resveratrol has a dual effect on the growth of S180 cell. Resveratrol is able to inhibit the proliferation of S180 cells by arresting the cell cycle at high concentrations. Regulating the levels of cell cycle regulatory proteins might be involved in the Resveratrol-induced cell cycle arrest.