小檗碱对实验大鼠糖脂代谢的影响

目的探讨小檗碱对实验大鼠胰岛素敏感性和血糖血脂的影响。方法实验大鼠分7组：普食对照组和小檗碱治疗组，高脂对照组和小檗碱治疗组，高脂饮食与极小剂量链脲佐菌素(STZ)诱导的糖尿病对照组，糖尿病小檗碱治疗组和二甲双胍治疗组。疗程为5周。以糖耐量试验评估胰岛功能，以胰岛素耐量试验中的降糖率检测胰岛素敏感性。结果(1)小檗碱使糖尿病鼠的降糖率升高48％，疗效较二甲双胍为好；小檗碱使高脂鼠的胰岛素敏感因子升高78％，延缓了普食鼠降糖率的下降；(2)小檗碱显著降低了高脂大鼠的空腹血糖和负荷血糖；(3)小檗碱使普食鼠、高脂鼠和糖尿病鼠的血清游离脂肪酸分别下调14％、24％和20％，使高脂鼠的血清甘油三酯降低57％。结论小檗碱有显著的胰岛素增敏和降低血清游离脂肪酸的作用，并能降低高脂大鼠的血糖。

Effects of berberine on glucose and lipid metabolism in animal experiment

Objective To investigate the effects of berberine on the insulin sensitivity, the blood glucose and the blood lipid with experimental animals Methods Seven groups of rats were used: routine diet control, routine diet plus berberine treatment, high fat control, high fat plus berberine treatment, diabetes control, diabetes treated by berberine and diabetes treated by metformin The diabetic rats were induced by high fat diet and a very small dose streptozotocin All rats were treated for 5 weeks Islet function was evaluated by glucose tolerance test Insulin sensitivity was detected by the glucose lowering rate (GLR) of insulin tolerance test Results (1) The GLRs of diabetic rats treated by berberine were elevated by 48%, which was better than that of metformin The insulin sensitive indexI of high fat rats treated by berberine was increased by 78% The descent of GLRs of routine diet rats was delayed by berberine too; (2) Fasting blood glucose and loading blood glucose were lowered by berberine significantly; (3) Serum free fatty acids (FFA) of routine diet rats, high fat rats and diabetic rats were decreased by 14%, 24% and 20% respectively regarding the effect of berberine Serum triglyceride of high fat rats treated by berberine was decreased by 57% in the presence of berberine Conclusions Berberine has remarkable insulin sensitizing and serum FFA lowering effects It still has a hypoglycemic effect on high fat rats