首页 | 本学科首页   官方微博 | 高级检索  
     


Performance Characteristics of Real-Time PCRs for African Swine Fever Virus Genome Detection—Comparison of Twelve Kits to an OIE-Recommended Method
Authors:Jutta Pikalo  Tessa Carrau  Paul Deutschmann  Melina Fischer  Kore Schlottau  Martin Beer  Sandra Blome
Affiliation:Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany; (J.P.); (T.C.); (P.D.); (M.F.); (K.S.); (M.B.)
Abstract:
African swine fever (ASF) is a major threat to pig production, and real-time PCR (qPCR) protocols are an integral part of ASF laboratory diagnosis. With the pandemic spread of ASF, commercial kits have risen on the market. In Germany, the kits have to go through an approval process and thus, general validation can be assumed. However, they have never been compared to each other. In this study, 12 commercial PCR kits were compared to an OIE-recommended method. Samples representing different matrices, genome loads, and genotypes were included in a panel that was tested under diagnostic conditions. The comparison included user-friendliness, internal controls, and the time required. All qPCRs were able to detect ASFV genome in different matrices across all genotypes and disease courses. With one exception, there were no significant differences when comparing the overall mean. The overall specificity was 100% (95% CI 87.66–100), and the sensitivity was between 95% and 100% (95% CI 91.11–100). As can be expected, variability concerned samples with low genome load. To conclude, all tests were fit for purpose. The test system can therefore be chosen based on compatibility and prioritization of the internal control system.
Keywords:African swine fever virus   laboratory diagnosis   commercial real-time PCR   performance   sensitivity   specificity
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号