麻疹中和试验对酶联免疫吸附试验的质量控制探讨

目的探讨酶联免疫吸附试验(ELISA)检测麻疹IgG抗体的质量控制方法。方法2003年对3个年龄组的294份ELISA法检测麻疹IgG抗体后的血清,用中和试验加以证实。结果两种方法相关,但差异较大,相关系数0.423~0.684,阳性符合率64.76%~98.24%。结论排除两种试验方法本身敏感性的差异,麻疹ELISA-IgG试剂盒质量的稳定性、操作方法的精确性等问题值得关注,必须确保血清学检测方法标准化。

Research on the Quality Control of Both Measles Neutralization Test and ELISA Assay

Objective The methodology of quality control to detect levels of measles IgG antibody by ELISA assay is discussed. Methods 294 samples of sera collected in three age groups in 2003 and tested for measles IgG antibody by ELISA assay were verified by the method of neutralization test. Results The two assays were relative,but a big difference existed (relative coefficients:0.423-0.684,positive coincidence rates:64.76%-98.24%). Conclusions Without regard to inherent diversity of sensitivity of two methods,certain problems such as the stability of measles ELISA-IgG kits and the accuracy of operation methods should be considered to ensure the quality of disease surveillance.The provincial center for disease control and prevention should play an important role in the quality control to standardize the testing method of serology.