低密度脂蛋白受体基因多态性及其与慢性丙型肝炎的相关性

目的探讨我国汉族正常人群及慢性丙型肝炎(chronicviralhepatitisC,CHC)患者低密度脂蛋白受体(LDL-R)基因外显子12中HincⅡ多态性的分布及其与慢性丙型肝炎之间的关系。方法采用聚合酶链反应结合限制性片段长度多态性(PCR-RFLP)的方法,对84例慢性丙型肝炎病毒(HCV)感染者和72名健康献血员进行LDL-R基因HincⅡ多态性检测。结果在HCV感染组和健康对照组、慢性丙型肝炎组和HCV相关肝硬化组、丙氨酸转氨酶(ALT)正常组和ALT升高组,以及HCVRNA阴性组和HCVRNA阳性组之间,LDL-R基因外显子12HincⅡ多态性分布差异无统计学意义。结论人群LDL-R基因外显子12中存在着HincⅡ多态性位点,但该多态性位点可能与慢性HCV感染无相关性。

Association between polymorphism of human low-density lipoprotein receptor gene and chronic viral hepatitis C in Chinese patients

Objective To detect HincⅡ polymorphism in low-density lipoprotein receptor(LDL-R) gene,and to study its relation to chronic viral hepatitis C(CHC) in the Han nationality of China.Methods Polymerase chain reaction restriction fragment length polymorphisms(PCR-RFLP) method was used to detect HincⅡ polymorphism of the LDL-R gene in 84 patients infected with HCV and 72 healthy blood donors.Results There was no statistically significant differences in HincⅡ polymorphism of the LDL-R gene exon 12 between patients infected with HCV and healthy controls,between patients with chronic viral hepatitis C and those with HCV associated cirrhosis,between patients with normalized serum ALT and those with abnormal ALT level,and between HCV RNA negative and positive patients.Conclusion There is HincⅡ polymorphism in the LDL-R gene exon 12 in the Han natiancity poputation,but it may not be involved in the chronic viral hepatitis C.