酪蛋白磷酸肽钙磷复合体对黏性放线菌黏附影响的实验研究

目的：研究酪蛋白磷酸肽钙磷复合体（casein phosphopeptide-amorphic calcium phosphate,CPP-ACP）对黏性放线菌黏附的影响,进一步探讨CPP-ACP的防龋机制。方法：采用唾液包被的羟磷灰石（saliva-coated hydroxyapatite,S-HA）形成实验性膜的体外黏附实验模型,用不同浓度（0.5%～5.0%（W/V））CPP-ACP处理S-HA,定量观察黏性放线菌在S-HA上的黏附情况。结果：黏性放线菌对经各实验浓度的CPP-ACP处理后的S-HA黏附能力明显下降,黏附量（CPM）随CPP-ACP浓度的升高而降低,且黏附抑制率均达100%。结论：CPP-ACP对黏性放线菌在S-HA上的黏附具有抑制作用,随CPP-ACP浓度的升高其抑制作用增强。

EXPERIMENTAL STUDY OF EFFECTS OF CASEIN PHOSPHOPEPTIDE-AMORPHIC CALCIUM PHOSPHATE ON ADHERENCE OF ACTINOMYCES VISCOSUS

Objective： To investigate the effects of casein phosphopeptide-amorphic calcium phosphate（CPP-ACP） on the adherence of actinomyces viscosus,then evaluate the possibility of CPP-ACP as an effective biological anti-caries agent.Methods： The hydroxyapatite（HA） beads were coated by saliva to form saliva-coated hydroxyapatite（S-HA）,as the adhesion model in vitro.Actinomyces viscosus was chosen as the experimental strain,and radiolabeled with 3H-thymidine.S-HA was treated with different concentrations（0.5%～5.0%（W/V）） of CPP-ACP.The number of 3H-thymidine labeled bacteria attached to S-HA was counted by scintillation counting.Results： CPP-ACP inhibited the adherence of actinomyces viscosus to S-HA effectively.With the concentration of CPP-ACP increasing,the CPM value of experimental groups dropped（P〈0.01）,and all the inhibition rate of adherence reached 100%.Conclusion： CPP-ACP can inhibit the adherence of actinomyces viscosus to S-HA significantly.With the concentration of CPP-ACP increasing,the adhesive inhibition enhances.