富血小板血浆PRP对骨髓基质细胞BMSCs碱性磷酸酶活性和总蛋白含量的影响

目的 :体外观察富血小板血浆 (Platelet -RichPlasma ,PRP)对自体骨髓基质细胞 (BoneMarrowStro malCells ,BMSCs)碱性磷酸酶活性和总蛋白含量的影响。方法 :利用免疫组化染色 ,酶动力学法和考马斯亮蓝法探讨PRP对BMSCs碱性磷酸酶活性和总蛋白含量的影响。结果 :PRP组和矿化诱导组碱性磷酸酶染色强阳性 ,对照组为阴性 ;碱性磷酸酶活性测定 ,PRP组明显高于对照组 (P <0 .0 1) ,但与矿化诱导组比较 ,无统计学意义 (P >0 .0 5 ) ;细胞总蛋白含量PRP组也明显高于对照组 (P <0 .0 1) ,但略低于矿化诱导组 ,两实验组比较无统计学差别 (P>0 .0 5 )。结论 :PRP能促进骨髓基质细胞碱性磷酸酶活性 ,并提高细胞总蛋白含量。

Influence of platelet-rich plasma (PRP) on alkaline phosphate activity and total protein concent of bone marrow stromal cells

Objective:In vitro to evaluate the effects of PRP on alkaline phosphate (ALP) activity and total protein content of BMSCs.Method: Immunochemical staining, kinetic method and Coomassie brilliant blue staining were used in this study.Result:The PRP group and the osteogenic-differentiating medium group showed strongly positive immunochemical staining while the control group showed negative. Similar to staining above, the alkaline phosphate activity means were significantly different between the PRP group and the control group (P<0.01) and there was no difference between the PRP group and the osteogenic-differentiating medium group (P>0.05). As to total protein content, even though the content of PRP group was lower than that of osteogenic-differentiating medium group, the statistical significance existed between the two experimental groups and the control group. Conclusion: PPR promotes alkaline phosphate activity and total protein content toward bone marrow stromal cells.