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| 野西瓜多糖诱导人肝癌HepG2细胞凋亡的实验研究 | |
| 引用本文： | 季宇彬,东方,高世勇,邹翔. 野西瓜多糖诱导人肝癌HepG2细胞凋亡的实验研究[J]. 中草药, 2008, 39(9): 1364-1367 |
| 作者姓名： | 季宇彬 东方 高世勇 邹翔 |
| 作者单位： | 哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,黑龙江,哈尔滨,150076;国家教育部抗肿瘤天然药物工程研究中心,黑龙江,哈尔滨,150076 |
| 基金项目： | 黑龙江省研究生创新科研项目，黑龙江省自然科学基金 |
| 摘 要： | 目的观察野西瓜多糖诱导人肺癌HepG2细胞凋亡作用。方法通过MTT法测定野西瓜多糖对HepG2细胞的细胞毒作用;倒置显微镜观察野西瓜多糖对HepG2细胞形态的影响;AO/EB双染,激光共聚焦扫描显微镜观察野西瓜多糖对HepG2细胞形态的影响;PI染色法,流式细胞术检测细胞凋亡率。结果MTT结果显示野西瓜多糖对HepG2细胞有抑制作用,IC50为471.53mg/L;倒置显微镜、激光共聚焦扫描显微镜下观察细胞形态,表现为细胞皱缩、碎裂、甚至出现凋亡小体;流式细胞术检测到野西瓜高剂量组凋亡率为74.926%。结论野西瓜多糖具有诱导HepG2细胞凋亡的作用。 |
| 关 键 词： | 野西瓜多糖 HepG2细胞 凋亡 |
| 收稿时间： | 2008-04-25 |
Apoptosis induced by Capparis spionosa poIysaccharide in human HepG2 |
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| JI Yu-bin,DONG Fang,GAO Shi-yong and ZOU Xiang. Apoptosis induced by Capparis spionosa poIysaccharide in human HepG2[J]. Chinese Traditional and Herbal Drugs, 2008, 39(9): 1364-1367 | |
| Authors: | JI Yu-bin DONG Fang GAO Shi-yong ZOU Xiang |
| Affiliation: | Postdoctoral Research Station of Institute of Materia Medica, Research Center of Life Science and Environment Science, Harbin University of Commerce, Harbin 150076, China;Postdoctoral Research Station of Institute of Materia Medica, Research Center of Life Science and Environment Science, Harbin University of Commerce, Harbin 150076, China;Postdoctoral Research Station of Institute of Materia Medica, Research Center of Life Science and Environment Science, Harbin University of Commerce, Harbin 150076, China;Postdoctoral Research Station of Institute of Materia Medica, Research Center of Life Science and Environment Science, Harbin University of Commerce, Harbin 150076, China |
| Abstract: | Objective To investigate the apoptosis of Capparis spionosa polysaccharide(CSPS)on human HepG2.Methods MTT was adopted to determine if CSPS had cytotoxic effect to HepG2.The alteration of HepG2's morphology was observed by invert microscope.Morphology of HepG2 changed with dosage of CSPS was detected by AO/EB and laser confocal scanning microscope.Flow cytometry(FCM)was used to detect the apoptosis index with PI labeling method.Results The result of MTT showed that CSPS could inhibit the growth of HepG2,IC50 was 471.53 mg/L;The morphology of cell was observed by invert microscope and laser confocal scanning microscope in cell collapsed,break to pieces,even appearance of apoptosis body.The apoptosis rate in high dose group was 74.926%,detected by FCM.Conclusion This consequence indicates that CSPS could induce human HepG2 apoptosis. |
| Keywords: | Capparis spionosa L.polysaccharide(CSPS) HepG2 apoptosis |
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