DNA prime followed by protein boost enhances neutralization and Th1 type immunity against FMDV |
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Authors: | Jin Huali Kang Youmin Xiao Chong Zhu Kaichun Ma Yijie Xie Qifa Ma Junwu Xie Qingge He Cheng Yang Zhansheng Sun Zhi Zhang Xinyu Chen Mingyong Zhang Fuchun Wang Bin |
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Affiliation: | State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China. |
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Abstract: | Prime-boost strategy has been exhibited its potency to enhance immune responses, which would be important to the success to develop a vaccine against the foot-and-mouth disease virus (FMDV). An eukaryotic expression construct encoding the FMDV capsid VP1 protein with a recombinant VP1 protein or a commercial FMDV vaccine were tested in the prime-boost strategy in mice and cattle trials. The levels of induced specific antibodies, T cell proliferations, and DTH activities were significantly higher in the prime-boost groups than in those vaccinated with DNA, protein or FMDV vaccine alone. More importantly, the levels of neutralizing antibodies in the former groups were significantly higher than others and could last for at least four months in cattle trials. This study suggests that the prime-boost strategy significantly improves the effective immunity and may provide a longer protection against FMDV infection. |
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