食管癌患者外周血CD4^＋CD25^＋Foxp3^＋调节性T细胞检测及其临床意义

目的:通过检测食管癌患者外周血中CD4 CD25 Foxp3 调节性T细胞的变化,观察其与临床及病理特征之间的关系,探讨其临床意义。方法:采用流式细胞术检测30例食管癌患者及16例健康志愿者外周血单个核细胞中CD4 CD25 Foxp3 T细胞占CD4 T细胞的比例;收集食管癌患者的临床及病理资料,分析研究Treg细胞变化与临床及病理特征之间的关系。结果:流式细胞术检测显示食管癌患者外周血PBMC中CD4 CD25 Foxp3 T细胞占CD4 T细胞的比例为(3.72±1.39)%,高于对照组(1.15±0.36)%,P<0.01;食癌管患者外周血中Treg细胞比例与患者的年龄、性别、肿瘤的位置无明显相关性(P>0.05),而与淋巴结转移情况及TNM分期有明显的相关性(P<0.05)。结论:CD4 CD25 Foxp3 调节性T细胞可能在食管癌免疫耐受中发挥重要作用,检测其比例对于判断食管癌的病期及预后有一定价值。

Detection and clinical significance of CD4+CD25+Foxp3+regulatory T cells in peripheral blood of esophageal cancer patients

Objective:To find out the change of CD4 CD25 Foxp3 regulatory T cells in peripheral blood of esophageal cancer patients,the relationship between clinical data and pcorholozical characteristics,and to study their clinical significance.Methods: Flow cytometry was used to analyze the proportion of CD4 CD25 Foxp3 T cells/CD4 T cells in peripheral blood mononuclear cells(PBMCs) from 30 patients with esophageal cancer and 16 healthy peoples.Clinical data and pathological characteristics of cancer patients were collected,and t-test was used to analyze the relationship between the proportion of Treg cells in PBMC and clinical data and pathological characteristics. Results: The proportion of CD4 CD25 Foxp3 T cells/CD4 T cells in PBMC from esophageal cancer patients was significantly higher than that of controls(P<0.01).The proportion of Treg cells in PBMC has no significant difference with sex,age,location of tumor,category of pathology and the infiltrating depth of tumor(P>0.05).The proportion of Treg cells in PBMC of cancer patients was correlated significantly with lymph node metastasis and stages of TNM(P<0.05). Conclusion: Treg may have an important role in immune tolerance of esophageal cancer.Detection of Treg cells have clinically value on evaluation of development and prognosis of esophageal cancer.