| 改良羊水染色体的制备技术 |
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| 引用本文： | 曾爱群,陈全娘,冯英,林雨,李雄.改良羊水染色体的制备技术[J].广州医学院学报,2001,29(4):42-43. |
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| 作者姓名： | 曾爱群 陈全娘 冯英 林雨 李雄 |
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| 作者单位： | 广州医学院第一附属医院妇产科, |
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| 摘 要： | 目的：寻找一种简便，快速，效果稳定的羊水细胞染色体的制备方法。方法：总结19年来我们遗传室羊水细胞培养的工作经验。结果和结论：通过增加培养液中L－谷氨酰胺的含量以及应用Hepes，可使羊水细胞生长良好，而短时间高浓度的秋水侧素可获得较多的染色体分裂象。
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| 关 键 词： | 羊水细胞 染色体 制备技术 改良 |
| 文章编号： | 1008-1836(2001)04-0042-02 |
| 修稿时间： | 2001年9月10日 |
A Modified Technique for the Preparation of Chromosome from Amniotic-fluid Cells |
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| ZENG Ai-qun,CHEN Quan-niang,FENG Ying,et al.A Modified Technique for the Preparation of Chromosome from Amniotic-fluid Cells[J].Academic Journal of Guangzhou Medical College,2001,29(4):42-43. |
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| Authors: | ZENG Ai-qun CHEN Quan-niang FENG Ying |
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| Abstract: | ve: To establish a simple,rapid and stable technique for the preparation of chromosome from amniotic -fluid cells. Methods: The experience in the cultivation of Amniotic fluid cells in the past 19 years in our genetics laboratory was summed up. Results and Conclusions: By increasing the amount of L-glutamine in culture medium and using Hepes,the amniotic fluid cells grew better. High concentration of colchicine in a short time period could achieve more chromosome mitosising phase. |
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| Keywords: | amniotic-fluid cell chromosome |
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