| 幽门螺杆菌调控胃上皮细胞基因表达的机制 |
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| 引用本文： | 李璟妍,汤燕,王瑛,王华,陈永昌.幽门螺杆菌调控胃上皮细胞基因表达的机制[J].江苏大学学报(医学版),2006,16(1):13-15. |
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| 作者姓名： | 李璟妍 汤燕 王瑛 王华 陈永昌 |
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| 作者单位： | 1. 江苏大学,医学院, 江苏 镇江 212001
2. 江苏大学,医学技术学院, 江苏 镇江 212001 |
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| 基金项目： | 江苏大学校科研和教改项目;江苏大学校科研和教改项目 |
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| 摘 要： | 目的：在基因转录调节水平上研究幽门螺杆菌生物活性物质调控胃上皮细胞基因表达的机制。方法：人胃上皮癌细胞株SGC-7901采用常规方法培养，幽门螺杆菌菌株NCTC11637采用马血清琼脂培养基培养，收获后用超声粉碎法提取生物活性物质，基因转录采用报告基因方法分析。结果：幽门螺杆菌提取物使SRE反式激活的基因转录增加（P〈0．01），而对CRE反式激活的基因转录没有明显影响（P〉0．01）。结论：幽门螺杆菌生物活性物质通过顺式转录调控元件SRE调控细胞基因表达。
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| 关 键 词： | 幽门螺杆菌 胃上皮细胞 基因表达 |
| 文章编号： | 1671-7783（2006）01-0013-03 |
| 收稿时间： | 2005-09-19 |
| 修稿时间： | 2005年9月19日 |
Mechanism of Helicobacter pylori Induced Gene Expression in the Gastric Epithelial Cells |
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| LI Jing-yan,TANG Yan,WANG Ying,WANG Hua,CHEN Yong-chang.Mechanism of Helicobacter pylori Induced Gene Expression in the Gastric Epithelial Cells[J].Journal of Jiangsu University Medicine Edition,2006,16(1):13-15. |
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| Authors: | LI Jing-yan TANG Yan WANG Ying WANG Hua CHEN Yong-chang |
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| Institution: | 1. School of Medicine, 2. School of Medical Technology. Jiangsu University, Zhenjiang Jiangsu 212001, China |
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| Abstract: | Objective: The experiment was designed to study the mechanism of Helicobacter pylori(Hp) induced gene expression in the gastric epithelium cells at the transduction level.Methods: Human gastric epithelial cancer cell line SGC-7901 was cultured with general methods.Hp line NCTC11637 was cultured on horse serum agarose culture plate.The extract of Hp was obtained by sonication.Gene expression was analyzed by report-gene assay.Results: Hp extract significantly increased SRE-driven gene expression but had no effect on CRE-driven gene expression.Conclusion: Biological active substance from Hp regulates gene expression through SRE cis-element. |
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| Keywords: | Helicobacter pylori Gastric epithelial cell Gene expression |
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