| 银染法观察铜绿假单胞菌生物被膜变化初探 |
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| 引用本文： | 孔晋亮,刘晓岚,陈一强,王立赞,闫萍,温红侠.银染法观察铜绿假单胞菌生物被膜变化初探[J].济宁医学院学报,2007,30(1):27-29. |
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| 作者姓名： | 孔晋亮 刘晓岚 陈一强 王立赞 闫萍 温红侠 |
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| 作者单位： | 1. 广西医科大学第一附属医院
2. 济宁医学院病理生理学教研室 |
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| 摘 要： | 目的探讨银染法观察细菌生物被膜在药物作用前后变化的可靠性。方法建立细菌生物被膜,分别用银染法和扫描电镜进行观察生物被膜的变化。结果银染后普通光学显微镜观察和扫描电镜观察对生物被膜变化的观察结果完全相符。结论用银染法观察细菌生物膜的变化便捷、可靠。
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| 关 键 词： | 生物被膜 银染法 铜绿假单胞菌 |
| 收稿时间： | 2006-12-05 |
| 修稿时间： | 2006-12-05 |
Obseruation the Pseudomsnas Aeruginosa Biofilm With Siluer Staining Method |
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| KONG Jinliang , LIU Xiaolan , CHEN Yiqiang , et al.Obseruation the Pseudomsnas Aeruginosa Biofilm With Siluer Staining Method[J].Jounal of Jinning Medical College,2007,30(1):27-29. |
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| Authors: | KONG Jinliang LIU Xiaolan CHEN Yiqiang |
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| Institution: | Department of Respiratory Medicine, First Affiliated Hospital, Guangxi Medical University |
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| Abstract: | Objective To investigate the reliability doserving pseudomonas aeruginosa biofilm with silver nitrate staining.Methods Establish pseudomonas aeruginosa biofilm model,adopting silver staining method and scanning electron microscopy to observe the variation of biofilm.Results Observation with general optical microscope after silver staining to appraise biofolm is identical with that using scanning electron microscope.Conclusion Silver staining method used to observe the variation of biofilm is reliable and simple. |
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| Keywords: | Biofilm silver nitrate staining Pseudomonas aeruginosa |
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