正畸应力作用下牙龈肌成纤维细胞表达的初步研究

目的 初步探讨正畸应力加载后牙龈肌成纤维细胞的表达情况。方法 选取8例正畸应力加载后需要拔除牙牙龈组织,以应力加载前拔除牙牙龈组织为对照,进行α-平滑肌肌动蛋白（α-SMA）、Ⅰ型胶原、Ⅲ型胶原免疫组织化学染色。然后进行测量和统计分析。结果 正畸应力加载前牙龈组织内Ⅰ型胶原、Ⅲ型胶原免疫组化染色阳性,α-SMA除血管上皮外,其余为阴性表达。正畸应力加载后,Ⅰ型胶原、Ⅲ型胶原表达明显上升,差异有统计学意义（P<0.05）;α-SMA在牙龈组织内出现阳性表达,与加力前差异有统计学意义（P<0.05）。结论 正畸应力加载后,牙龈组织内肌成纤维细胞出现表达,其可能在正畸牙术后复发中发挥一定作用。

Expression of myofibroblast in gingival after orthodontic loading

Objective To examine the expression of myofibroblast in gingival after orthodontic loading. Methods Eight patients were selected as experimental group and treated with orthodontic force for 4 months. Ten patients were selected as the control group, were not loaded. The gingival protein expressions of collagen typeⅠ, collagen type Ⅲ, α-smooth muscle actin (α-SMA) were evaluated by immunohistochemistry method. Results Positive expressions of collagen typeⅠ, collagen type Ⅲ were founded, while no positive staining for α-SMA in the gingival tissue except vascular epithelium before loading. In experimental group, collagen type I and collagen type Ⅲ were increased after orthodontic loading (P<0.05), the expression of α-SMA was detected and statistically significant (P<0.05). Conclusion The myofibroblast exists in gingival tissue after orthodontic loading, and it may be concerned with orthodontic teeth relapse.