基于太赫兹时域光谱技术的名贵动物药材龟甲的真伪品鉴别

目的： 建立一种利用太赫兹时域光谱和多种化学计量学方法鉴别龟甲真伪的新方法。方法： 选用正品乌龟和伪品红耳彩龟的腹甲进行实验，先用经典的性状鉴别、显微鉴别方法比较两种样品的典型性状特征，后利用太赫兹时域光谱仪测得样品的光谱信息，对样品谱图进行平滑处理和基线校准，并运用化学计量方法进行数据分析以区分正品和伪品龟甲。结果： 性状鉴别结果显示完整正、伪品龟甲样品仅有细微差异，且其碎片或粉末基本无差异；显微鉴别未在两种样品中发现明显的特征差异性结构；两种样品的太赫兹时域光谱曲线图经快速傅里叶变换滤波器平滑滤波处理、正交偏最小二乘法判别分析处理后呈现显著差异。结论： 利用太赫兹时域光谱技术结合正交偏最小二乘法判别分析可以实现龟甲真伪品的精准鉴别。

Authenticity identification of famous and precious animal medicine tortoiseshell by terahertz time-domain spectroscopy#br#

Objective To establish a new method for identification of true and false tortoiseshells by terahertz time domain spectroscopy and stoichiometry. Methods The samples of authentic tortoiseshell and fake tortoiseshell from the red eared slider were selected for further experiments. The typical characteristics of the two samples were compared by classical character identification and microscope identification methods, and then the spectral information of the samples was measured by terahertz time domain spectrometer. The sample spectra were smooth processed and baseline calibrated, and the data were analyzed by stoichiometric method to distinguish the authentic tortoiseshells from the fake tortoiseshells. Results The results of character identification showed that there were only slight differences between intact and fake tortoiseshell samples, and little differences in fragments or powder. Microscopic identification of the two samples did not find obvious characteristic difference structure. After fast Fourier transform filter and orthogonal partial least square discriminant analysis, the terahertz time domain spectral curves of the two samples showed significant differences. Conclusion Terahertz time domain spectroscopy combined with orthogonal partial least square discriminant analysis could achieve accurate identification of true and false tortoiseshells.