Characterization of genetic determinants involved in antibiotic resistance in Aeromonas spp. and fecal coliforms isolated from different aquatic environments |
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| Affiliation: | 1. Research Unit of Analysis and Process Applied to the Environmental – APAE Higher Institute of Applied Sciences and Technology Mahdia - University of Monastir, Tunisia;2. Laboratory of Chemical Residues and Contaminants, Direction of Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium;3. Laboratoire Traitement et recyclage des eaux, Centre de Recherche et Technologie des Eaux, BorjCedria, Tunisia;4. Hygiene Laboratory/Toxicology Unit Hedi CHAKER Hospital, Sfax, Tunisia;5. Université Bretagne Sud, FRE CNRS 3744, IRDL, 56017 Vannes, France;1. South China Sea Resource Exploitation and Protection Collaborative Innovation Center, School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, China;2. Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China;3. MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China;4. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510275, China;1. Department of Environmental Management and Toxicology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154 Benin City 300001, Nigeria;2. SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa;3. Applied Microbial Processes & Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154 Benin City 300001, Nigeria;4. Environmental, Public Health and Bioresource Microbiology Research Group, Department of Basic Sciences, Benson Idahosa University, PMB 1100, Benin City, Nigeria;5. Department of Biological Sciences, Microbiology Division, Faculty of Agriculture Science and Technology, North West University, Mafikeng Campus, Private Bag X2046, Mmabatho 2375 South Africa |
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| Abstract: | Aeromonas spp. and fecal coliforms, two abundant and cultivable bacterial populations that can be found in water ecosystems, might substantially contribute to the spread of antibiotic resistance. We investigated the presence and spread of transposons (elements that can move from one location to another in the genome), integrons (structures able to capture and incorporate gene cassettes) and resistance plasmids in strains isolated from polluted and unpolluted water. We recovered 231 Aeromonas and 250 fecal coliforms from water samplings with different degrees of pollution (hospital sewage, activated sludge of a wastewater treatment plant, river water before and after treatment and water from an alpine lake). Sixteen Aeromonas spp. and 22 fecal coliforms carried intI, coding for the site-specific integrase of class 1 integrons, while 22 Aeromonas spp. and 14 fecal coliforms carried tnpA, the transposase gene of the Tn3-family of replicative transposons. The majority of intI and tnpA-positive strains were phenotypically resistant to at least four antibiotics. Integrons and transposons were mainly located on mobilizable plasmids.Our results did not detect common mobile structures in the two populations and therefore relativize the role played by Aeromonas spp. as vectors of antimicrobial resistance determinants between water and commensal gut bacteria. |
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| Keywords: | Class 1 integrons Resistance plasmids Aquatic environment |
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