多聚双磷酸腺苷核糖聚合酶-1基因与儿童肥胖和非酒精性脂肪肝的关联

目的研究多聚双磷酸腺苷核糖聚合酶-1(PARP1)基因rs1136410多态性与儿童青少年肥胖和非酒精性脂肪肝(NAFLD)的关系,为阐明儿童青少年肥胖和NAFLD的发病机制与制定早期预防策略提供依据。方法选取北京市2030名7~18岁中小学生进行身体测量,并对部分学生进行肝脏B超检查。利用基质支持的激光释放/电离飞行时间质谱分析(MALDI-TDF MS)对PARP1基因rs1136410多态性进行检测。采用多元Logistic回归分析rs1136410多态性与儿童青少年肥胖或NAFLD的关系。结果2030名中小学生中,检出肥胖者705名。在所有样本中,PARP1基因rs1136410的G和A的等位基因频率分别为43.86%和56.14%。校正性别、年龄和研究人群后发现,PARP1基因rs1136410多态性每增加1个A等位基因,发生肥胖的风险增加为1.17倍(95%CI=1.02~1.33,P=0.03),发生非酒精性脂肪肝的风险增加为1.43倍(95%CI=1.11~1.85,P=0.01);进一步校正BMI后,rs1136410多态性与NAFLD无相关性(P=0.70)。结论PARP1基因rs1136410多态性与儿童青少年肥胖和NAFLD有相关性,该基因多态性对NAFLD的作用可能是通过影响BMI实现。

Association between poly (ADP-ribose) polymerase-1 polymorphism and obesity and non-alcoholic fatty liver disease among children and adolescents

Objective To study the relationship between rs1136410 polymorphism in poly(ADP-ribose) polymerase-1 gene(PARP1) with obesity and non-alcoholic fatty liver disease(NAFLD) in children and adolescents, and to provide a reference for demonstrating the mechanism of obesity and NAFLD of children and adolescents and making the early prevention strategies. Methods In total, 2 030 children and adolescents aged 7-18 years old were recruited. Anthropometric measurements were performed. Liver B-ultrasound detection were performed in a subgroup. The matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TDF MS) was used for genotyping the rs1136410 polymorphism. Logistic regression was used to analyze the relationship between rs1136410 polymorphism and obesity or NAFLD in children and adolescents. Results Totally, 1 325 subjects were in non-obese group and 705 subjects were in obese group. The frequency of G allele and A allele were 43.86% and 56.14%. After adjusting for gender, age, and study population, the risk of obesity increased by 1.17 times for each additional A allele of rs1136410 polymorphism(OR=1.17, 95%CI=1.02-1.33, P=0.03). And the risk of NAFLD increased by 1.43 times for each additional A allele of rs1136410 polymorphism(OR=1.43, 95%CI=1.11-1.85, P=0.01). After further adjustment for BMI, rs1136410 polymorphism was not associated with NAFLD(P=0.70). Conclusion The rs1136410 polymorphism in PARP1 gene is associated with obesity and NAFLD in children and adolescents. The effect of the gene polymorphism on NAFLD is mediated by BMI.