Bacterial associates of Hyalesthes obsoletus (Hemiptera: Cixiidae), the insect vector of bois noir disease,with a focus on cultivable bacteria |
| |
| Institution: | 1. Department of Entomology, Newe Ya''ar Research Center, ARO, Israel;2. Department of Biotechnology, ORT Braude College, Israel;3. Shamir Research Institute, Israel;4. Ohallo College, Israel;5. Extension Service, Ministry of Agriculture, Israel;6. Department of Biology, University of Victoria, Victoria, Canada;7. Northern R&D, Galilee Technology Center (MIGAL), Israel;1. University of Tunis El Manar, Faculty of Sciences of Tunis, LR 206 Micro-Organisms and Bio-molecules Actives, 2092, Tunisia;2. Charles Nicolle Hospital, Hepato-Gastro-Enterology department, Tunis, Tunisia;3. Military Hospital of Tunis, Laboratory of Immunology, Tunis, Tunisia;4. Department of Virology, Amiens University Medical Centre, Amiens, France;5. Virology Research Unit, EA 4294, Jules Verne University of Picardie, Amiens, France;1. Shamir Research Institute, University of Haifa, P.O. Box 97, Qatzrin, Israel;2. Tel Hai College, Upper Galilee, 12208, Israel;3. Department of Biotechnology Engineering, ORT Braude College, P.O. Box 78, 21982, Karmiel, Israel;4. Faculty of Civil & Environmental Engineering, Division of Environmental, Water & Agricultural Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel;5. Water Industries Engineering Department, Achi Racov Engineering School, Kinneret College on the Sea of Galilee, D.N. Emek Ha’Yarden, 15132, Israel;6. BioCastle Water Technologies Ltd., Israel;7. Department of Chemical Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel;1. Department of Clinical Laboratory, Nantong Third Hospital Affiliated to Nantong University, Nantong 212006, Jiangsu, China;2. Medical Risk Management Section, Third Medical Centre, Chinese PLA (People''s Liberation Army) General Hospital, Beijing 100039, China;3. First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning, China;4. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;5. School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, China |
| |
| Abstract: | The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is an important vector of phytoplasma diseases in grapevine. In the current study, the bacterial community compositions of symbionts of this insect were examined. Two dominant bacterial lineages were identified by mass sequencing: the obligate symbiont Candidatus Sulcia, and a facultative symbiont that is closely related to Pectobacterium sp. and to BEV, a cultivable symbiont of another phytoplasma vector, the leafhopper Euscelidius variegatus. In addition, one bacterium was successfully isolated in this study – a member of the family Xanthomonadaceae that is most closely related to the genus Dyella. This Dyella-like bacterium (DLB) was detected by FISH analysis in H. obsoletus guts but not ovaries, and its prevalence in H. obsoletus increased during the fall, suggesting that it was acquired by the host through feeding. We found that DLB inhibits Spiroplasma melliferum, a cultivable relative of phytoplasma, suggesting that it is a potential candidate for biological control against phytoplasma in grapevines. |
| |
| Keywords: | Insect symbiont Planthopper Phytoplasma vector Bacterial community composition Grapevine-yellows Gut symbiont |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
